Class 1 integrons and tetracycline resistance genes in Alcaligenes, Arthrobacter, and Pseudomonas spp. isolated from pigsties and manured soil
Class 1 integrons and tetracycline resistance genes in Alcaligenes, Arthrobacter, and Pseudomonas spp. isolated from pigsties and manured soil
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DOI：
10.1128/aem.71.12.7941-7947.2005
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发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Sandvang, D
中科院分区：
文献类型：
--
作者：
Agerso, Y;Sandvang, D
四环素耐药（Tc-r）基因和I类整合子（in-1）的存在下，和他们的能力cotransfer进行了研究，在Tc-r革兰氏阴性菌（185株）和革兰氏阳性菌（72株）从丹麦农田和猪舍。分离株属于大肠杆菌，肠杆菌属，节杆菌属，产碱菌属，假单胞菌属，和谷氨酸棒杆菌。对257株分离株进行in-1筛选。还对81株革兰氏阴性分离株进行了Tc-r基因泰特（A）、泰特（B）和泰特（C）筛选，对所有（n = 72）革兰氏阳性分离株进行了泰特（33）筛选。14个（7%）的土壤菌株和11个（25%）的猪圈菌株中含有-1。除一个来自猪舍的革兰氏阴性分离株含有泰特（B）外，所有含有泰特基因的分离株也含有in-1。所有具有in-1的革兰氏阳性分离株也含有泰特（33）。没有分离株含有一个以上的泰特基因。检测了in-l阳性分离株对选定抗菌药物的耐药性，并显示对3至9种药物的耐药性。过滤器交配实验表明，共转移的Tc-r和I类整合子从土壤分离到大肠杆菌和/或恶臭假单胞菌。我们的结论是，土壤细菌密切接触粪便或猪舍环境，因此可能有重要的作用，在水平传播的电阻。在食用动物生产中使用四环素类抗生素不仅可能增加Tc-r，而且可能增加细菌的多药耐药性（由泰特基因和in-1的存在引起）。
The presence of tetracycline resistance (Tc-r) genes and class I integrons (in-1), and their ability to cotransfer were investigated in Tc-r gram-negative (185 strains) and gram-positive (72 strains) bacteria from Danish farmland and pigsties. The isolates belonged to the groups or species Escherichia coli, Enterobacter spp., Arthrobacter spp., Alcaligenes spp., Pseudomonas spp., and Corynebacterium glutamicum. The 257 isolates were screened for in-1. Eighty-one of the gram-negative isolates were also screened for the Tc-r genes tet(A), tet(B), and tet(C), and all (n = 72) gram-positive isolates were screened for tet(33). Fourteen (7%) of the soil isolates and eleven (25%) of the pigsty isolates contained in-1. All isolates that contained tet genes also contained in-1, except one gram-negative isolate from a pigsty that contained tet(B). All gram-positive isolates with in-1 also contained tet(33). No isolates contained more than one tet gene. The in-l-positive isolates were tested for resistance to selected antimicrobial agents and showed resistance to three to nine drugs. Filter-mating experiments showed cotransfer of Tc-r and class I integrons from soil isolates to Escherichia coli and/or Pseudomonas putida. We conclude that soil bacteria in close contact to manure or pigsty environment may thus have an important role in horizontal spread of resistance. Use of tetracyclines in food animal production may increase not only Tc-r but also multidrug resistance (caused by the presence tet genes and in-1) in bacteria.