Kinetic Analysis and Modeling of Daptomycin Batch Fermentation by Streptomyces roseosporus
Kinetic Analysis and Modeling of Daptomycin Batch Fermentation by Streptomyces roseosporus
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DOI：
10.1007/s12010-010-9053-6
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发表时间：
2011-02-01
影响因子：
3
通讯作者：
Caiyin, Qinggele
中科院分区：
文献类型：
--
作者：
Lu, Wenyu;Fan, Jinghua;Caiyin, Qinggele
In this study, Streptomyces roseosporus was subjected to helium-neon (He-Ne) laser (632.8 nm) irradiation to improve the production ability of extracellular antibiotic daptomycin. Under the optimum irradiation dosage of 18 mW for 22 min, a stable positive mutant strain S. roseosporus LC-54 was obtained. The maximum A21978C (daptomycin is a semisynthetic antimicrobial substance derived from the A21978C complex) yield of this mutant strain was 296 mg/l, which was 146% higher than that of the wild strain. The mutant strain grew more quickly and utilized carbohydrate sources more efficiently than the wild strain. The batch culture kinetics was investigated in a 7 l bioreactor. The logistic equation for growth, the Luedeking-Piret equation for daptomycin production, and Luedeking-Piret-like equations for carbon substrate consumption were established. This model appeared to provide a reasonable description for each parameter during the growth phase and fitted fairly well with the experiment data.
