Genome-wide identification of tissue-specific enhancers in the Ciona tadpole
Genome-wide identification of tissue-specific enhancers in the Ciona tadpole
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DOI：
10.1073/pnas.052024999
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发表时间：
2002-05-14
影响因子：
11.1
通讯作者：
Levine, M
中科院分区：
文献类型：
--
作者：
Harafuji, N;Keys, DN;Levine, M
在转基因后生动物胚胎的背景下，不到100个顺式调控dna被表征。在这里，我们研究了在海鞘中进行组织特异性增强子全基因组搜索的可行性。在lacZ报告基因的5′处，随机放置138个平均大小为1.7 kb的基因组DNA片段。11个lacZ融合基因在蝌蚪期红豆胚中表现出局部表达模式。这些转基因中至少有五种似乎含有真正的组织特异性增强子，这些增强子直接在脑泡、神经管、原始粘附器官、脊索和尾表皮中表达。其中一个增强子位于Distalless （G-DII-A）附近，概括了内源性表达模式的大多数方面，包括神经源性外胚层最前部的局部表达。我们讨论了创建Ciona基因组的调控图谱的前景，其中每个增强子被鉴定为每个基因。
Less than 100 cis-regulatory DNAs have been characterized in the context of transgenic metazoan embryos. Here we investigate the feasibility of conducting a genome-wide search for tissue-specific enhancers in the ascidian Ciona intestinalis. A total of 138 random genomic DNA fragments with an average size of 1.7 kb were separately placed 5' of a lacZ reporter gene. Eleven of the lacZ fusion genes displayed localized patterns of expression in tadpole-stage Ciona embryos. At least five of these transgenes appear to contain bona fide tissue-specific enhancers that direct expression in the cerebral vesicle, neural tube, primordial adhesive organ, notochord, and tail epidermis. One of the enhancers maps near Distalless (G-DII-A) and recapitulates most aspects of the endogenous expression pattern, including localized expression in the anterior-most regions of the neurogenic ectoderm. We discuss the prospects of creating a regulatory atlas of the Ciona genome, whereby every enhancer is identified for every gene.