Preliminary Quality Criteria of Citrate-Protected Gold Nanoparticles for Medicinal Applications
Preliminary Quality Criteria of Citrate-Protected Gold Nanoparticles for Medicinal Applications
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DOI：
10.1021/acsanm.8b00192
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发表时间：
2018-04
期刊：
影响因子：
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通讯作者：
Jia-Jia Shen-Jia;Pingbo Zhang;Feng Zheng;Huan Chen;Wei Chen;Ya Ding;X. Xia
中科院分区：
文献类型：
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作者：
Jia-Jia Shen-Jia;Pingbo Zhang;Feng Zheng;Huan Chen;Wei Chen;Ya Ding;X. Xia
金纳米粒子（GNP）是纳米医学中研究和使用最广泛的材料之一，但其医学应用没有商业或国家质量标准。这已成为金基纳米材料临床应用转化的瓶颈。为了解决这一问题，本研究选择了最常用的柠檬酸盐还原GNPs作为示范，进行质量标准研究。通过改变氯金酸与柠檬酸三钠的摩尔比（1：1 ~ 1：5），调整胶体金的粒径和主要成分。采用X射线光电子能谱（XPS）、电感耦合等离子体-原子发射光谱（ICP-AES）和光动力催化法测定了还原态Au 0和残余Au 3+的组成，采用核磁共振（1H NMR）和非水滴定法测定了上清液中残余柠檬酸盐及其氧化产物的组成。正常人的细胞活力...
Gold nanoparticles (GNPs) are one of the most widely studied and utilized materials in nanomedicine, but there is no business or national quality standard for their medicinal applications. This has become the bottleneck in the translation of clinical applications of gold-based nanomaterials. To solve this issue, the most frequently used citrate-reduced GNPs were selected as demonstration in this study to conduct quality standard research. The different molar ratios of chloroauric acid to trisodium citrate in preparation were changed, from 1:1 to 1:5, to adjust the particle size and main ingredients in gold colloids. The compositions of reduced Au0 and residual Au3+ were determined by X-ray photoelectron spectroscopy (XPS), inductively coupled plasma–atomic emission spectroscopy (ICP-AES), and photodynamic catalysis, while the residual citrate and its oxidation products in supernatants were detected by nuclear magnetic resonance (1H NMR) spectroscopy and nonaqueous titration. Cell viability of human normal...