Isolation of a unique benzothiophene-desulphurizing bacterium, Gordona sp. strain 213E (NCIMB 40816), and characterization of the desulphurization pathway
Isolation of a unique benzothiophene-desulphurizing bacterium, Gordona sp. strain 213E (NCIMB 40816), and characterization of the desulphurization pathway
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DOI：
10.1099/00221287-144-9-2545
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发表时间：
1998-09-01
期刊：
影响因子：
--
通讯作者：
McRoberts, A
中科院分区：
文献类型：
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作者：
Gilbert, SC;Morton, J;McRoberts, A
Gordona sp.菌株213E(NCIMB 40816)在以果糖为碳源和能源、苯并噻吩硫化物为唯一硫源的无机盐培养基上生长。在生长过程中积累了一种酚类化合物，如添加吉布斯试剂产生蓝色所表明的那样。因此，该途径类似于红球菌的二苯并噻吩(DBT)脱硫途径。菌株IGTS8，以DBT为唯一硫源，在生长过程中积累2-羟基联苯。培养基乙酸乙酯萃取物产生代谢物苯并[c][1，2]氧杂环己烷(BTHO)，苯并[c][1，2]氧杂硫杂环己烷(BcOTO)，2-(2‘-羟基苯基)乙烷1-醛(HPEal)和苯并呋喃(BFU)，推测BTH的硫化途径为：BTH->；BTHO-->；BTHO2-->；HPESi-->；HPEAl，HPESi-IS(Z)-(2’-羟基苯基)E L-硫酸盐，BcOTO的稳定水溶液形式。结果表明，HPEal是213E菌株生长过程中积累在培养基中的Gibb试剂反应性酚类化合物，而BFU的存在是由于HPEal在乙酸乙酯提取过程中发生部分缩合所致。Gordona sp.213e菌株不能在以果糖为碳源和能源、DBT为唯一硫源的矿盐中生长。BTH-脱硫-活性细胞(以BTH为唯一硫源培养)不能对DBT进行脱硫。同样，菌株IGTS8不能以BTH为唯一硫源生长，而以DBT为唯一硫源生长的菌株IGTS8的DBT-脱硫活性细胞也不能对BTH进行脱硫。根据DBT-和BTH-脱硫反应的基本化学差异，讨论了这种交叉反应的缺乏。
Gordona sp. strain 213E (NCIMB 40816) grew in pure culture in a mineral salts medium containing fructose as a source of carbon and energy, and benzothiophene (BTH) as the sole source of sulphur. During growth a phenolic compound accumulated, as indicated by the production of a blue colour an addition of Gibb's reagent. Therefore this pathway is analogous to the dibenzothiophene (DBT) desulphurization pathway of Rhodococcus sp. strain IGTS8, in which 2-hydroxybiphenyl accumulates during growth with DBT as the sole sulphur source. Ethyl acetate extraction of the culture medium yielded the metabolites benzothiophene S-oxide (BTHO), benzothiophene S,S-dioxide (BTHO2), benzo[c][1,2]oxathiin 6-oxide (BcOTO), 2-(2'-hydroxyphenyl)ethan 1-al (HPEal) and benzofuran (BFU), The deduced pathway for BTH desulphurization is BTH --> BTHO --> BTHO2 --> HPESi- --> HPEal, HPESi- is (Z)-2-(2'-hydroxyphenyl)ethen l-sulphinate, the stable aqueous-solution form of BcOTO. It was concluded that HPEal was the Gibb's-reagent-reactive phenolic compound which accumulated in the culture medium of strain 213E during growth, and that the presence of BFU was due to partial condensation of HPEal during the ethyl acetate extraction procedure. Gordona sp, strain 213E was unable to grow in a mineral salts medium containing fructose as a source of carbon and energy and DBT as the sole sulphur source. BTH-desulphurization-active cells (grown using BTH as sole sulphur source) were unable to desulphurize DBT. Likewise Rhodococcus sp, strain IGTS8 was unable to grow using BTH as the sole sulphur source, and DBT-desulphurization-active cells of strain IGTS8 (grown using DBT as sole sulphur source) were unable to desulphurize BTH. This absence of crossreactivity is discussed in terms of fundamental differences in the chemistry of the DBT- and BTH-desulphurization reactions.