ASSEMBLY OF SNRNP-CONTAINING COILED BODIES IS REGULATED IN INTERPHASE AND MITOSIS - EVIDENCE THAT THE COILED BODY IS A KINETIC NUCLEAR-STRUCTURE
ASSEMBLY OF SNRNP-CONTAINING COILED BODIES IS REGULATED IN INTERPHASE AND MITOSIS - EVIDENCE THAT THE COILED BODY IS A KINETIC NUCLEAR-STRUCTURE
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DOI：
10.1083/jcb.120.4.841
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发表时间：
1993-02-01
影响因子：
7.8
通讯作者：
LAMOND, AI
中科院分区：
文献类型：
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作者：
CARMOFONSECA, M;FERREIRA, J;LAMOND, AI
卷曲体（CB）是剪接snRNP集中的核细胞器。虽然有时观察到CB与核仁周边相关，但它们不含5S或28S rRNA或U3 snoRNA。这反对CB在rRNA成熟或转运中发挥作用，如先前所建议的。我们在这里提出的证据表明，CBs的动力学结构，并表明，含snRNP的CBs的形成是受调控的间期和有丝分裂。卷曲体抗原，p80 coilin，存在于所有类型的细胞研究，即使当CB并不突出。细胞生长温度的变化可以诱导CB形成的显著变化，而细胞内p80卷曲蛋白水平没有伴随变化。在有丝分裂期间，CB分解，与p80卷曲蛋白的有丝分裂特异性磷酸化一致。卷曲蛋白是一种磷蛋白，在有丝分裂过程中至少有两个额外的位点被磷酸化。CBs在子核中经过一个未被检测到的滞后期后进行改革。因此，CB是动态的核细胞器，我们提出，剪接snRNP与CB的循环相互作用可能是重要的，它们参与前mRNA在哺乳动物细胞中的加工或运输。
Coiled bodies (CBs) are nuclear organelles in which splicing snRNPs concentrate. While CBs are sometimes observed in association with the nucleolar periphery, they are shown not to contain 5S or 28S rRNA or the U3 snoRNA. This argues against CBs playing a role in rRNA maturation or transport as previously suggested. We present evidence here that CBs are kinetic structures and demonstrate that the formation of snRNP-containing CBs is regulated in interphase and mitosis. The coiled body antigen, p80 coilin, was present in all cell types studied, even when CBs were not prominent. Striking changes in the formation of CBs could be induced by changes in cellular growth temperature without a concomitant change in the intracellular p80 coilin level. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin. Coilin is shown to be a phosphoprotein that is phosphorylated on at least two additional sites during mitosis. CBs reform in daughter nuclei after a lag period during which they are not detected. CBs are thus, dynamic nuclear organelles and we propose that cycling interactions of splicing snRNPs with CBs may be important for their participation in the processing or transport of pre-mRNA in mammalian cells.