Human herpesviruses in the cornea
Human herpesviruses in the cornea
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DOI：
10.1136/bjo.84.6.563
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发表时间：
2000-06-01
影响因子：
4.1
通讯作者：
Hart, CA
中科院分区：
文献类型：
--
作者：
Kaye, SB;Baker, K;Hart, CA
目的：探讨培养、免疫组织化学(IHC)、聚合酶链式反应(PCR)和原位杂交法(ISH)检测穿透性角膜移植患者角膜中单纯疱疹病毒(HSV-I)的敏感性和特异性。目的比较单纯疱疹病毒1型(HSV-1)与水痘检测病毒(VZV)、巨细胞病毒(CMV)和EB病毒(EBV)在角膜中的感染情况。方法：对110例单纯疱疹病毒性角膜炎(HSK)患者和58例非疱疹角膜病变患者的角膜进行免疫组化、聚合酶链式反应(IHC)、原位杂交(ISH)和培养检测。结果HSV-1 DNA和抗原在单纯疱疹病毒性角膜炎(HSK)患者角膜中的阳性率分别为82%和74%，在无HSK病史患者的角膜中分别为22%和15%。PCR法和IHC法的敏感性分别为82%和74%，特异性分别为78%和85%。单纯疱疹病毒1型DNA和抗原在最后一次HSK发作至手术时间较短的患者的角膜中出现的频率更高，数量也更多。聚合酶链式反应与间接免疫组化的符合率为71%。单纯疱疹病毒1型(HSV-1)的分离率为2%。用ISH没有检测到潜伏期相关的转录本。在有HSK病史的患者的角膜中发现VZV DNA或抗原的证据明显更多(P
Aims-To determine the sensitivity and specificity of culture, immunohistochemistry (IHC), the polymerase chain reaction (PCR), and in situ hybridisation (ISH) for detecting herpes simplex virus (HSV-I) in the cornea of patients undergoing penetrating keratoplasty. To compare the incidence of HSV-1 in the cornea with that of varicella tester virus (VZV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV).Methods-The corneas of 110 patients, 52 with a documented history of herpes keratitis (HSK) and 58 with non-herpetic corneal disease, were investigated using IHC, PCR, ISH, and culture.Results-HSV-1 DNA and antigen were detected in 82% and 74% respectively, of corneas of patients with HSK and in 22% and 15% of corneas of patients with no history of HSK. The sensitivity of PCR and IHC was 82% and 74% with a specificity of 78% and 85%, respectively. HSV-1 DNA and antigen were found more frequently and in increased amounts in corneas of patients with a short interval between their last attack of HSK and surgery. There was a good correlation between PCR and IHC in 71%. HSV-1 was isolated by culture in 2%. Latency associated transcripts were not detected using ISH. Evidence of VZV DNA or antigen was found significantly more frequently in the corneas of patients with a history of HSK (p