Effect of femtosecond laser energy level on corneal stromal cell death and inflammation.
Effect of femtosecond laser energy level on corneal stromal cell death and inflammation.
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DOI：
10.3928/1081597x-20090917-08
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Wilson SE
中科院分区：
文献类型：
--
作者：
de Medeiros FW;Kaur H;Agrawal V;Chaurasia SS;Hammel J;Dupps WJ Jr;Wilson SE
在兔模型中分析飞秒激光能量水平变化对角膜基质细胞死亡和角膜瓣形成后炎症细胞流入的影响。根据用于皮瓣创建的能量水平，将18只家兔分为3个不同的组（每组6只动物）。选择三种不同的能量水平用于薄片和侧切：2.7 μJ（高能）、1.6 μJ（中能）和0.5 μJ（低能），使用60 KHz II型飞秒激光（IntraLase）。每只兔子的另一只眼睛作为对照。在术后24小时的时间点，所有家兔均被安乐死，并分别用末端尿苷脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）测定和单核细胞标志物CD 11b的免疫细胞化学分析角巩膜缘的细胞死亡和炎性细胞流入水平。高能组（31.9±7.1 [平均标准误差（SEM）2.9]）与中间皮瓣相比，中央皮瓣中的TUNEL阳性细胞显著更多。（22.2±1.9 [SEM 0.8]，P=.004）、低（17.9±4.0 [SEM 1.6]，P≤.001）和对照眼（0.06±0.02 [SEM 0.009]，P≤.001）组。中、低能量组TUNEL阳性细胞也显著多于对照组（P≤.001）。中间和低能量水平之间的差异不显著（P= 0.56）。对于高能量、中能量、低能量和对照组，皮瓣边缘处CD 11b阳性细胞/400×视野的平均值分别为26.2±29.3（SEM 12.0）、5.8±4.1（SEM 1.7）、1.7±4.1（SEM 1.7）和0.0±0.0（SEM 0.0）。只有中等能量组显示出比对照眼更多的炎性细胞（P= 0.015），这很可能是由于眼睛之间的差异。当飞秒激光用于创建角膜瓣时，更高的能量水平触发更大的细胞死亡。飞秒激光能量水平越高，观察到角膜炎性细胞浸润越大。
To analyze the effects of variations in femtosecond laser energy level on corneal stromal cell death and inflammatory cell influx following flap creation in a rabbit model. Eighteen rabbits were stratified in three different groups according to level of energy applied for flap creation (six animals per group). Three different energy levels were chosen for both the lamellar and side cut: 2.7 μJ (high energy), 1.6 μJ (intermediate energy), and 0.5 μJ (low energy) with a 60 KHz, model II, femtosecond laser (IntraLase). The opposite eye of each rabbit served as a control. At the 24-hour time point after surgery, all rabbits were euthanized and the corneoscleral rims were analyzed for the levels of cell death and inflammatory cell influx with the terminal uridine deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and immunocytochemistry for monocyte marker CD11b, respectively. The high energy group (31.9±7.1 [standard error of mean (SEM) 2.9]) had significantly more TUNEL-positive cells in the central flap compared to the intermediate (22.2±1.9 [SEM 0.8], P=.004), low (17.9±4.0 [SEM 1.6], P≤.001), and control eye (0.06±0.02 [SEM 0.009], P≤.001) groups. The intermediate and low energy groups also had significantly more TUNEL-positive cells than the control groups (P≤.001). The difference between the intermediate and low energy levels was not significant (P=.56). The mean for CD11b-positive cells/400× field at the flap edge was 26.2±29.3 (SEM 12.0), 5.8±4.1 (SEM 1.7), 1.7±4.1 (SEM 1.7), and 0.0±0.0 (SEM 0.0) for high energy, intermediate energy, low energy, and control groups, respectively. Only the intermediate energy group showed statistically more inflammatory cells than control eyes (P=.015), most likely due to variability between eyes. Higher energy levels trigger greater cell death when the femtosecond laser is used to create corneal flaps. Greater corneal inflammatory cell infiltration is observed with higher femtosecond laser energy levels.