Transcriptomic investigation reveals a physiological mechanism for Beauveria bassiana to survive under linoleic acid stress
Transcriptomic investigation reveals a physiological mechanism for Beauveria bassiana to survive under linoleic acid stress
复制标题
DOI：
10.1016/j.isci.2023.106551
复制
发表时间：
2023-04-12
期刊：
影响因子：
5.8
通讯作者：
Ying, Sheng-Hua
中科院分区：
文献类型：
--
作者：
Hou, Jia;Zhang, Hao;Ying, Sheng-Hua
In integrated pest management program (IPM), the compatibility of mycoinsecti-cides with bioactive fungicides [e.g., unsaturated fatty acids (UFAs)] has attracted more and more attention; however, the mechanisms underlying fungal resistance to UFAs remain largely unknown. In this study, Beauveria bassiana, an entomopa-thogenic fungus, was used to explore fungal responses to linoleic acid (LA). Genome-wide expression revealed the transcriptomic responses of fungal cells to LA in a stress-intensity-dependent manner. Enrichment analyses indicated that the up-regulated differentially expressed genes (DEGs) are associated with the metabolism of lipid and fatty acids. Notably, a lipid-droplet protein (BbLar1) maintains the intracellular homeostasis of fatty acids and is crucial to fungal tolerance to LA stress, which significantly contributes to fungal compati-bility with UFAs. Additionally, BbLar1 links the lipid droplets to global expression profiles in B. bassiana under LA stress. Our investigations provide an initial frame-work for improving the efficacy of insect pathogenic fungi in practical application.
