The effect of gold nanoparticles modified electrode on the glucose sensing performance
The effect of gold nanoparticles modified electrode on the glucose sensing performance
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DOI：
10.1063/1.4993334
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发表时间：
2017-07
影响因子：
5
通讯作者：
Z. A. Zulkifli;Nur Syafinaz Ridhuan;Noorhashimah Mohamad Nor;N. Zakaria;K. A. Razak
中科院分区：
文献类型：
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作者：
Z. A. Zulkifli;Nur Syafinaz Ridhuan;Noorhashimah Mohamad Nor;N. Zakaria;K. A. Razak
本文采用种子生长法制备了20 nm、30 nm、40 nm、50 nm和60 nm的胶体金纳米颗粒（AuNPs）。制备的AuNPs呈球形，大小均匀。AuNPs也以胶体形式很好地分散，这是由低多分散指数证明的。所得AuNPs用于修饰葡萄糖传感器电极。将生成的AuNPs沉积在氧化铟锡底物（ITO）上，然后将葡萄糖氧化酶（GOx）固定在其上。此后，Nafion被存入GOx/AuNPs/ITO。用循环伏安计（CV）研究了在15mm葡萄糖与0.01 M PBS混合条件下，aunps修饰电极对葡萄糖的电氧化作用。AuNPs的最佳尺寸为30 nm，光密度为3.0。AuNPs成功地用葡萄糖氧化酶（GOx）固定化，并被证明可以很好地作为葡萄糖传感器。基于Nafion/GOx/AuNPs/ITO的高电催化活性，通过在0.01 M磷酸缓冲液（PBS）溶液中改变葡萄糖溶液的浓度从2 mM到20 mM，进一步检测葡萄糖传感器的灵敏度。催化电流与葡萄糖浓度在2 ~ 20 mM范围内呈良好的线性关系。根据线性平方校准斜率计算的Nafion/GOx/AuNPs/ITO电极的灵敏度为0.909µA mM−1 cm−2，与其他已发表的研究结果相当。线性拟合实验数据给r平方0.991在0.9 V和2.03毫米的检测极限。这探测范围是充分的医用价值在人类正常的血糖水平监测血糖水平在4.4到6.6毫米的范围和糖尿病血糖水平高于7 mM.In这项工作,20 nm, 30 nm, 40 nm, 50和60 nm胶体金纳米粒子(AuNPs)使用种子生长法合成。制备的AuNPs呈球形，大小均匀。AuNPs也以胶体形式很好地分散，这是由低多分散指数证明的。所得AuNPs用于修饰葡萄糖传感器电极。将生成的AuNPs沉积在氧化铟锡底物（ITO）上，然后将葡萄糖氧化酶（GOx）固定在其上。此后，Nafion被存入GOx/AuNPs/ITO。用循环伏安计（CV）研究了在15mm葡萄糖与0.01 M PBS混合条件下，aunps修饰电极对葡萄糖的电氧化作用。AuNPs的最佳尺寸为30 nm，光密度为3.0。AuNPs成功地用葡萄糖氧化酶（GOx）固定化，并被证明可以很好地作为葡萄糖传感器。基于Nafion/GOx/AuNPs/ITO的高电催化活性，进一步通过改变葡萄糖浓度来检测葡萄糖传感器的灵敏度。
In this work, 20 nm, 30 nm, 40 nm, 50 nm and 60 nm colloidal gold nanoparticles (AuNPs) were synthesized using the seeding growth method. AuNPs produced had spherical shape with uniform size. The AuNPs also are well dispersed in colloidal form that was proven by low polydispersity index. The produced AuNPs were used to modify electrode for glucose sensor. The produced AuNPs were deposited on indium tin oxide substrate (ITO), followed by immobilization of glucose oxidase (GOx) on it. After that, Nafion was deposited on the GOx/AuNPs/ITO. Electrooxidation of glucose with AuNPs-modified electrode was examined by cyclic voltammeter (CV) in 15 mM glucose mixed with 0.01 M PBS. The optimum size of AuNPs was 30 nm with optical density 3.0. AuNPs were successfully immobilized with glucose oxidase (GOx) and proved to work well as a glucose sensor. Based on the high electrocatalytic activity of Nafion/GOx/AuNPs/ITO, the sensitivity of the glucose sensors was further examined by varying the concentration of glucose solution from 2 mM to 20 mM in 0.01 M phosphate buffer solution (PBS) solution. Good linear relationship was observed between the catalytic current and glucose concentration in the range of 2 mM to 20 mM. The sensitivity of the Nafion/GOx/AuNPs/ITO electrode calculated from the slope of linear square calibration was 0.909 µA mM−1 cm−2 that is comparable with other published work. The linear fitting to the experimental data gives R-square of 0.991 at 0.9 V and a detection limit of 2.03 mM. This detection range is sufficient to be medically useful in monitoring human blood glucose level in which the normal blood glucose level is in the range of 4.4 to 6.6 mM and diabetic blood glucose level is above 7 mM.In this work, 20 nm, 30 nm, 40 nm, 50 nm and 60 nm colloidal gold nanoparticles (AuNPs) were synthesized using the seeding growth method. AuNPs produced had spherical shape with uniform size. The AuNPs also are well dispersed in colloidal form that was proven by low polydispersity index. The produced AuNPs were used to modify electrode for glucose sensor. The produced AuNPs were deposited on indium tin oxide substrate (ITO), followed by immobilization of glucose oxidase (GOx) on it. After that, Nafion was deposited on the GOx/AuNPs/ITO. Electrooxidation of glucose with AuNPs-modified electrode was examined by cyclic voltammeter (CV) in 15 mM glucose mixed with 0.01 M PBS. The optimum size of AuNPs was 30 nm with optical density 3.0. AuNPs were successfully immobilized with glucose oxidase (GOx) and proved to work well as a glucose sensor. Based on the high electrocatalytic activity of Nafion/GOx/AuNPs/ITO, the sensitivity of the glucose sensors was further examined by varying the concentration of glucose ...