Characterization of a novel bacteriophage specific to Exiguobacterium indicum isolated from a plateau eutrophic lake
Characterization of a novel bacteriophage specific to Exiguobacterium indicum isolated from a plateau eutrophic lake
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从高原富营养化湖泊中分离出的一种针对印度微小杆菌的新型噬菌体的表征
DOI：
10.1002/jobm.201800184
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发表时间：
2019
影响因子：
3.1
通讯作者：
Xiao Wei
中科院分区：
文献类型：
--
作者：
Zhang Shiying;Fan Cong;Xia Yunsheng;Li Mengke;Wang Yongxia;Cui Xiaolong;Xiao Wei
微小杆菌是一个多功能属，在工业和农业中具有潜力。尽管噬菌体对微小杆菌的利用有潜在影响，但尚未报道感染微小杆菌的噬菌体。 E. indicum EI9分离自中国高原富营养化湖泊滇池，能显着抑制铜绿微囊藻的生长。为了分离和表征感染微小杆菌的噬菌体（一种强毒噬菌体），从滇池水样中分离出特异性感染 E. indicum EI9 的 DCEIV-9。 DCEIV-9 产生微小、圆形、透明的斑块，直径为 0.5-1mm。电镜显示DCEIV-9是管病毒科的典型代表，具有二十面体头部（直径56纳米）和非收缩性尾部（长度163纳米）。基于一步生长曲线，确定了 20 分钟的潜伏期和 51 PFU/感染细胞的爆发大小。 DCEIV-9对50℃以上的温度敏感，更喜欢酸性环境。 DCEIV-9 对蛋白酶 K、氯仿、乙醇、Triton X-100 极其敏感，但对 SDS 不敏感。限制性内切酶分析表明DCEIV-9是一种双链DNA病毒。 DCEIV-9只能感染印度斑马鱼，表明其宿主范围较窄。 DCEIV-9是一个潜在的新物种。
Exiguobacterium is a versatile genus with potential in industry and agriculture. No bacteriophage that infects Exiguobacterium has been reported, despite its potential impacts on the utilization of Exiguobacterium. E. indicum EI9 was isolated from Dianchi Lake, a plateau eutrophic lake in China, which can significantly inhibit the growth of Microcystis aeruginosa. To isolate and characterize Exiguobacterium‐infecting bacteriophage, a virulent bacteriophage, DCEIV‐9 that specifically infects E. indicum EI9 was isolated from Dianchi lake water sample. DCEIV‐9 produced tiny, round, and clear plaques with 0.5–1 mm in diameter. Electron microscopy showed that DCEIV‐9 is a typical representative of the Siphoviridae, with an icosahedral head (56 nm in diameter) and a non‐contractile tail (163 nm in length). Based on a one‐step growth curve, latent period of 20 min and burst size of 51 PFU/infected cell were determined. DCEIV‐9 was sensitive to temperature over 50 °C and prefers acid environment. DCEIV‐9 was extremely sensitive to proteinase K, chloroform, ethanol, Triton X‐100 but not sensitive to SDS. Restriction endonucleases analysis indicated that DCEIV‐9 is a dsDNA virus. DCEIV‐9 can only infect E. indicum, indicates that it has a narrow host range. DCEIV‐9 is a potential new species.