Solution Viscosity‐Mediated Structural Control of Nanofibrous Sponge for RNA Separation and Purification
Solution Viscosity‐Mediated Structural Control of Nanofibrous Sponge for RNA Separation and Purification
复制标题
DOI：
10.1002/adfm.202112023
复制
发表时间：
2022-02
影响因子：
19
通讯作者：
Pan Cheng;Ke Liu;Yucai Wan;Wei Hu;Cancan Ji;Peng Huang;Qihao Guo;Jia Xu;Qin Cheng;Dong Wang
中科院分区：
文献类型：
--
作者：
Pan Cheng;Ke Liu;Yucai Wan;Wei Hu;Cancan Ji;Peng Huang;Qihao Guo;Jia Xu;Qin Cheng;Dong Wang
具有可控多孔通道结构和化学活性的工程 3D 纳米纤维整体材料有望用于快速有效地分离环境敏感的生物大分子（如 RNA）。在此，开发了通过调节纳米纤维分散体粘度的冷冻干燥方法，通过将聚乙烯醇-共-乙烯（PVA-共-PE）纳米纤维与分散体中的壳聚糖（CS）和聚乙烯亚胺（PEI）结合来制造具有微球、薄片和蜂窝3D结构的纳米纤维海绵（NFS）。在海绵中，蜂窝状纳米纤维海绵（HNFS）具有高度定向的开孔和最佳性能，包括在1 psi下的透水性为7.431 × 103 L h m−2，静态饱和容量为887.6 mg g−1，在流速为1 mm min−1时动态容量为763.2 mg g−1，机械鲁棒性优于报道的最先进的离子交换色谱材料。此外，HNFS 填充的色谱柱、漏斗柱和离心柱在从模型溶液和多种生物体中快速分离 RNA 且不降解方面表现出卓越的性能和广泛的适用性。它们归因于纳米纤维细胞壁表面的高度定向的开孔结构和高密度吸附配体。这项工作提供了一种简单的方法来设计具有多功能物理化学结构和出色性能的先进 NFS。
Engineering 3D nanofibrous monolithic materials with a controllable porous channel structure and chemical activity is promising for the rapid and efficient separation of environmentally sensitive biomacromolecules such as RNA. Herein, freeze‐drying method via regulating the viscosity of nanofiber dispersions is developed to fabricate nanofibrous sponges (NFSs) with 3D structures of microsphere, lamella, and honeycomb by combining poly(vinyl alcohol‐co‐ethylene) (PVA‐co‐PE) nanofibers with chitosan (CS) and polyethyleneimine (PEI) in dispersions. Among sponges, a honeycomb nanofibrous sponge (HNFS) possesses highly oriented open‐cells and optimal performance, including water permeability of 7.431 × 103 L h m−2 at 1 psi, static saturated capacity of 887.6 mg g−1, dynamic capacity of 763.2 mg g−1 at a flow rate of 1 mm min−1, and mechanical robustness, superior to reported state‐of‐the‐art ion‐exchange chromatography materials. Furthermore, HNFS‐packed chromatographic, funnel, and centrifugal columns present exceptional performance and broad applicability in rapid‐separation of RNA from model solution and diverse organisms without degradation. They are attributed to the highly oriented open‐cell structure and high‐density adsorption ligands on the surface of nanofibrous cell walls. This work provides a facile approach to design advanced NFSs with versatile physicochemical structure and outstanding performance.