Commonly used Saccharomyces cerevisiae strains (e.g. BY4741, W303) are growth sensitive on synthetic complete medium due to poor leucine uptake
Commonly used Saccharomyces cerevisiae strains (e.g. BY4741, W303) are growth sensitive on synthetic complete medium due to poor leucine uptake
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DOI：
10.1111/j.1574-6968.2007.00798.x
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发表时间：
2007-08-01
影响因子：
2.1
通讯作者：
Engelberg, David
中科院分区：
文献类型：
--
作者：
Cohen, Ruth;Engelberg, David
据报道，一些广泛使用的酿酒酵母实验室菌株(如W303、BY4741)对所有20个氨基酸的合成培养基[如合成完整(SC)培养基或补充微量培养基]都很敏感。为了确定这种意想不到的敏感性的分子基础，我们筛选了一个基因组文库，并鉴定了三个基因，当过度表达时，将细胞从这种表型中拯救出来。其中两个“拯救”基因BAP2和TAT]与亮氨酸的运输有关，一个基因LEU2与亮氨酸的合成有关，这表明对SC介质的敏感性与Leu2突变有关。当这些敏感菌株生长在合成的完整培养基上时，它们似乎不能运输亮氨酸。在分析基因筛查和对这些菌株进行的其他实验的结果时，应该考虑leu2突变的这种影响。
It is reported that some of the widely used laboratory strains of Saccharomyces cerevisiae (e.g. W303, BY4741) are sensitive to synthetic media containing all 20 amino acids [e.g. synthetic complete (SC) medium or supplemented minimal medium]. To determine the molecular basis for this unexpected sensitivity, a genomic library was screened and three genes were identified that, when overexpressed, rescue cells from this phenotype. Two of the 'rescuing' genes, BAP2 and TAT], are related to transport of leucine, and one, LEU2, to synthesis of leucine, showing that sensitivity to SC medium is associated with the leu2 mutation. The sensitive strains seem incapable of transporting leucine when grown on synthetic complete media. This effect of the leu2 mutation should be taken into consideration when analyzing the results of genetic screens and other experiments performed with these strains.