Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops
Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops
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DOI：
10.1111/j.1574-6941.2001.tb00841.x
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发表时间：
2001-07-01
影响因子：
4.2
通讯作者：
Young, JPW
中科院分区：
文献类型：
--
作者：
Daniell, TJ;Husband, R;Young, JPW
我们利用小亚基核糖体 RNA 基因的差异来鉴定丛枝菌根真菌类群，这些真菌在英国北约克郡周围耕地中生长的植物根部定殖中非常活跃。从四个耕地和四个作物品种中收集根样品，使用引物 NS31 和 AM1 通过聚合酶链反应从单个植物中扩增真菌序列。对产物进行克隆，并根据 HinfI 或 RsaI 的限制性模式对 303 个克隆进行分类；随后对 72 个进行了测序。定殖以球囊霉属物种为主，仅占两种序列类型的优势。是密切相关的。有证据表明，就定植水平和存在的序列类型而言，定植存在季节性变化。附近林地的真菌多样性比之前报道的要低得多。 (C) 2001 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
We used differences in small subunit ribosomal RNA genes to identify groups of arbuscular mycorrhizal fungi that are active in the colonisation of plant roots growing in arable fields around North Yorkshire, UK. Root samples were collected From four arable fields and four crop species, fungal sequences were amplified from individual plants by the polymerase chain reaction using primers NS31 and AM1. The products were cloned and 303 clones were classified by their restriction pattern with HinfI or RsaI; 72 were subsequently sequenced. Colonisation was dominated by Glomus species with a preponderance of only two sequence types. which are closely related. There is evidence Fur seasonal variation in colonisation in terms of both level of colonisation and sequence types present. Fungal diversity was much lower than that previously reported for a nearby woodland. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights rsserved.