Antagonistic sensory cues generate gustatory plasticity in Caenorhabditis elegans
Antagonistic sensory cues generate gustatory plasticity in Caenorhabditis elegans
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DOI：
10.1038/sj.emboj.7600940
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发表时间：
2006-01-25
期刊：
影响因子：
11.4
通讯作者：
Jansen, G
中科院分区：
文献类型：
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作者：
Hukema, RK;Rademakers, S;Jansen, G
秀丽隐杆线虫对0.1 - 200 mM NaCl表现出化学吸引力，避免较高的NaCl浓度，并且在长期暴露于NaCl后避免其他有吸引力的NaCl浓度（味觉可塑性）。以往的研究表明，ASE和ASH感觉神经元主要介导的吸引和回避的NaCl，分别。在这里，我们表明，至少有四种感觉细胞类型，ASE，ASI，ASH，ADF，也许ADL之间的平衡，调节NaCl的反应。我们的研究结果表明，两个NaCl吸引信号通路存在，其中之一使用Ca 2 +/cGMP信号。此外，我们提供的证据表明，NaCl的吸引力是拮抗的G-蛋白信号在ASH神经元，这是脱敏的G-蛋白偶联受体激酶GRK-2。最后，对NaCl的反应由ASI和ADF神经元中的G蛋白信号传导、ASH中的第二G蛋白通路和暴露于体液的神经元中的cGMP信号传导来调节。
Caenorhabditis elegans shows chemoattraction to 0.1 - 200 mM NaCl, avoidance of higher NaCl concentrations, and avoidance of otherwise attractive NaCl concentrations after prolonged exposure to NaCl ( gustatory plasticity). Previous studies have shown that the ASE and ASH sensory neurons primarily mediate attraction and avoidance of NaCl, respectively. Here we show that balances between at least four sensory cell types, ASE, ASI, ASH, ADF and perhaps ADL, modulate the response to NaCl. Our results suggest that two NaCl-attraction signalling pathways exist, one of which uses Ca2+/cGMP signalling. In addition, we provide evidence that attraction to NaCl is antagonised by G-protein signalling in the ASH neurons, which is desensitised by the G-protein-coupled receptor kinase GRK-2. Finally, the response to NaCl is modulated by G-protein signalling in the ASI and ADF neurons, a second G-protein pathway in ASH and cGMP signalling in neurons exposed to the body fluid.