Effect of N-acy-L-homoserine lactones-like molecules from aerobic granules on biofilm formation by Escherichia coli K12
Effect of N-acy-L-homoserine lactones-like molecules from aerobic granules on biofilm formation by Escherichia coli K12
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DOI：
10.1016/j.biortech.2012.12.043
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发表时间：
2013-02-01
影响因子：
11.4
通讯作者：
Yu, Han-qing
中科院分区：
文献类型：
--
作者：
Ren, Ting-ting;Li, Xiao-yan;Yu, Han-qing
本文研究了好氧颗粒在膜分隔生物反应器中产生群体感应（QS）分子的过程。用大肠杆菌K12进行的流室（FC）试验表明，颗粒比活性污泥絮凝体更能诱导大肠杆菌细胞的附着生长，导致更多的细胞粘附并在FC盖玻片上形成生物膜。采用薄层色谱法，对颗粒污泥液相中酰基链短于10碳的n-酰基-l -同型丝氨酸内酯（AHLs）进行了检测。酸化乙酸乙酯从颗粒污泥上清液中提取有机物，促进大肠杆菌细胞在玻璃表面的粘附和生长。ahl样信号分子显然是由颗粒产生的，可能参与了废水处理过程中颗粒的形成和颗粒结构的维持。(C) 2012 Elsevier Ltd.版权所有。
A laboratory study was conducted to investigate the production of quorum sensing (QS) molecules by aerobic granules in membrane-partitioned bioreactor. Flow-chamber (FC) tests with Escherichia coli K12 demonstrated that granules induced more attached growth of E. coli cells than activated sludge flocs, leading to more cell adhesion and biofilm formation on the FC cover slide. Using the thin-layer chromatography, N-acy-L-homoserine lactones (AHLs) with acyl chains shorter than 10 carbons were detected in the liquid phase of granular sludge. Organic substances extracted with acidified ethyl acetate from the supernatant of granular sludge promoted the adhesion and growth of E. coli cells on the glass surface. AHL-like signal molecules were apparently produced by granules and might be involved in the formation of granules and the maintenance of granular structures during wastewater treatment. (C) 2012 Elsevier Ltd. All rights reserved.