Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
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DOI：
10.1002/prca.200900103
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发表时间：
2010-04
影响因子：
2
通讯作者：
Falcon-Perez, Juan M.
中科院分区：
文献类型：
--
作者：
Conde-Vancells, Javier;Rodriguez-Suarez, Eva;Gonzalez, Esperanza;Berisa, Agustin;Gil, David;Embade, Nieves;Valle, Mikel;Luka, Zigmund;Elortza, Felix;Wagner, Conrad;Lu, Shelly C.;Mato, Jose M.;Falcon-Perez, Juan M.
临床上迫切需要识别肝脏疾病的分子生物标记物，以便为诊断、预后和治疗提供信息。我们研究了从研究肝脏损伤的实验模型中获得的尿样中存在的尿小泡的蛋白质组，作为一种识别潜在的肝病生物标志物的方法。对高度纯化的外切体样尿囊泡进行的生化和蛋白质组学研究已经鉴定出28种以前未见报道的蛋白质，其中许多与疾病相关，例如普里恩相关蛋白。此外，在D-氨基半乳糖处理的大鼠的尿样中，我们检测到一些蛋白质严重减少，这些蛋白质通常在尿囊中清楚地检测到。最后，还鉴定了慢性肝损伤小鼠模型尿液小泡上的差异蛋白质含量。我们的结果肯定地证明，尿囊泡可以作为识别肝损伤的非侵入性生物标志物的来源。我们提出了一些蛋白，如CD26，CD81，SLC3A1和CD10，这些蛋白已经在一些分析的模型中发现在尿囊中差异表达，作为潜在的肝损伤生物标志物。
There is a compelling clinical imperative to identify discerning molecular biomarkers of hepatic disease in order to inform the diagnosis, prognosis and treatment. We have investigated the proteome of urinary vesicles present in urine samples obtained from experimental models for the study of liver injury, as an approach for identifying potential biomarkers for hepatic disease. The biochemical and proteomic characterization of highly purified exosome-like urinary vesicles has identified 28 proteins previously unreported in these vesicles, and many that have been previously associated with diseases, such as the prion-related protein. Furthermore, in urine samples from d-galactosamine-treated rats, a well-characterized experimental model for acute liver injury, we have detected a severe reduction in some proteins that normally are clearly detected in urinary vesicles. Finally, differential protein content on urinary vesicles from a mouse model for chronic liver injury has been also identified. Our results argue positively that urinary vesicles could be a source for identifying non-invasive biomarkers of liver injury. We proposed some proteins such as Cd26, Cd81, Slc3A1 and Cd10 that have been found to be differentially expressed in urinary vesicles from some of the analyzed models as potential biomarkers for liver injury.
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影响因子：
4.4
作者：
Slrnalley, David M.;Sheman, Nicholas E.;Theodorescu, Dan
通讯作者：
Theodorescu, Dan
影响因子：
13.6
作者：
Gonzales, Patricia A.;Pisitkun, Trairak;Knepper, Mark A.
通讯作者：
Knepper, Mark A.
影响因子：
3.6
作者：
KEPPLER, D;LESCH, R;DECKER, K
通讯作者：
DECKER, K
DOI：
10.1073/pnas.0308413101
发表时间：
2004-06-29
影响因子：
11.1
作者：
Fevrier, B;Vilette, D;Raposo, G
通讯作者：
Raposo, G
影响因子：
13.5
作者：
Martinez-Chantar, M. Luz;Vazquez-Chantada, Mercedes;Mato, Jose M.
通讯作者：
Mato, Jose M.