Isolation and characterization of a barley yellow stripe-like gene, HvYSL5.
Isolation and characterization of a barley yellow stripe-like gene, HvYSL5.
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DOI：
10.1093/pcp/pcr009
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发表时间：
2011-05
影响因子：
4.9
通讯作者：
Luqing Zheng;Miho Fujii;N. Yamaji;Akimasa Sasaki;M. Yamane;I. Sakurai;Kazuhiro Sato;J. Ma
中科院分区：
文献类型：
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作者：
Luqing Zheng;Miho Fujii;N. Yamaji;Akimasa Sasaki;M. Yamane;I. Sakurai;Kazuhiro Sato;J. Ma
黄条样转运蛋白（YSL）家族是寡肽转运蛋白（OPT）的一个新的亚家族，被认为与金属的摄取和长距离转运有关，但目前对其功能的研究还很少。在本研究中，我们分离出一个未知的成员的YSL家族，HvYSL 5，在大麦表达序列标签（EST）信息的基础上。HvYSL 5与铁-麦黄酸复合物的转运蛋白HvYS 1在氨基酸水平上有50%的同源性。启动子分析表明，HvYSL 5上游序列含有缺铁反应元件1和2（IDE 1和2）。HvYSL 5在根中表达，缺铁对表达有明显的诱导作用，而缺Zn、Cu、Mn等金属对表达无明显影响。空间分布研究表明，HvYSL 5基因在根的成熟区表达量较高，而在根尖表达量较低。而且，表达呈现日节律，上午最高，下午无表达。HvYSL 5定位于所有根细胞中，亚细胞定位分析表明HvYSL 5可能定位于囊泡中。HvYSL 5的敲低没有导致任何可检测的表型变化。虽然HvYSL 5的确切作用仍有待研究，但我们的研究结果表明，它参与了Fe或植物铁载体的瞬时储存。
Yellow stripe-like (YSL) family transporters, belonging to a novel subfamily of oligopeptide transporter (OPT), has been proposed to be involved in metal uptake and long-distance transport, but only a few of them have been functionally characterized so far. In the present study, we isolated an uncharacterized member of the YSL family, HvYSL5, in barley based on expressed sequence tag (EST) information. HvYSL5 shared 50% identity with HvYS1, a transporter for the ferric-mugineic acid complex, at the amino acid level. Promoter analysis showed that the HvYSL5 upstream sequence contains both iron deficiency response element 1 and 2 (IDE1 and 2). HvYSL5 was expressed in the roots and the expression was greatly induced by Fe deficiency, but not by deficiency of other metals including Zn, Cu and Mn. Spatial investigation showed that much higher expression of HvYSL5 was found in the mature zones of the roots, but not in the root tips. Furthermore, the expression showed a diurnal rhythm, being the highest in the morning, but with no expression in the afternoon. HvYSL5 was localized in all root cells, and subcellular localization analysis showed that HvYSL5 is likely to be localized in the vesicles. Knockdown of HvYSL5 did not result in any detectable phenotype changes. Although the exact role of HvYSL5 remains to be examined, our results suggest that it is involved in the transient storage of Fe or phytosiderophores.