Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats
Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats
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DOI：
10.1016/j.tvjl.2010.11.001
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发表时间：
2011-11-01
影响因子：
2.2
通讯作者：
Morimatsu, M.
中科院分区：
文献类型：
--
作者：
Ochiai, K.;Yoshikawa, Y.;Morimatsu, M.
在人类中，乳腺癌易感蛋白BRCA2基因的突变会影响其与重组酶RAD51的相互作用，并与癌症风险增加有关。这种相互作用通过BRCA2中的一系列8个BRC重复序列发生。使用单个BRC重复序列的哺乳动物双杂交试验表明，BRC6不与RAD51结合，而其他BRC重复序列与RAD51有强(BRC1、2和4)、中等(BRC8)或弱(BRC3、5和7)结合。在序列缺失突变实验中，当BRC1-8、BRC1-5和BRC1-3的C端BRC重复序列被移除时，结合强度增加。这些结果可能为深入了解BRCA2错义或截断突变在犬肿瘤中的影响提供依据。(C)2010爱思唯尔有限公司。保留所有权利。
In humans, mutations in the gene for the breast cancer susceptibility protein BRCA2 affect its interactions with the recombinase RAD51 and are associated with an increased risk of cancer. This interaction occurs through a series of eight BRC repeat sequences in BRCA2. A mammalian two-hybrid assay using individual BRC repeats demonstrated that BRC6 did not bind to RAD51, whereas there was strong (BRC1, 2 and 4), intermediate (BRC8), or weak (BRC3, 5 and 7) binding of other BRC repeats to RAD51. In serial deletion mutation experiments, binding strengths were increased when the C-terminal BRC repeat was removed from BRC1-8, BRC1-5 and BRC1-3. These results may provide an insight into the effects of missense or truncation mutations in BRCA2 in canine tumours. (C) 2010 Elsevier Ltd. All rights reserved.