Occurrence and phylogenetic analysis of Pseudanabaena sp. producing 2-methylisoborneol in drinking water source of South Korea
Occurrence and phylogenetic analysis of Pseudanabaena sp. producing 2-methylisoborneol in drinking water source of South Korea
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DOI：
10.1111/1758-2229.13031
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发表时间：
2021-11-27
影响因子：
3.3
通讯作者：
Byeon, Myeongseop
中科院分区：
文献类型：
--
作者：
Lee, Jung Eun;Yu, Mi Na;Byeon, Myeongseop
从7月到10月，我们在韩国的3个采样点调查了pseudoanabaena物种的丰度和单萜烯2-甲基异冰片醇（2-MIB）的浓度。为了确定饮用水水源中2-MIB发生的主要原因，我们对2-MIB合成酶基因进行了表征并进行了系统发育分析。蓝藻中以假蓝藻为优势菌群（68% ~ 100%）。在所有三个采样点，检测到2-MIB浓度与假藻细胞数量之间存在很强的正相关。对水样中分离的222个MIB序列进行系统发育分析，结果表明，所有的MIB克隆都与伪滨水藻MIB合成酶基因有关，表明汉江饮用水源中的2-MIB是由伪滨水藻产生的。利用2-MIB基因克隆，采用网络分析和非加权对群法结合算法均值分析，对2-MIB浓度和伪滨水藻丰度的时空变化进行了分析。网络分析显示，2-MIB基因克隆的时间相似性大于空间相似性。综上所述，我们的研究结果表明，pseudoanabaena是2-MIB的主要生产者。这些发现为饮用水水源的气味管理提供了重要信息。
We investigated the abundance of Pseudanabaena species and the concentration of the monoterpene 2-methylisoborneol (2-MIB) from July to October at three sampling sites in South Korea. To identify the main cause of 2-MIB occurrence in drinking water source, we characterized and performed a phylogenetic analysis of the 2-MIB synthase gene. Pseudanabaena was the dominant cyanobacterium (68%-100%) among the samples. At all three sampling sites, a strong positive correlation was detected between 2-MIB concentrations and Pseudanabaena cell numbers. A phylogenetic analysis of 222 MIB sequences isolated from the water samples showed that all of the clones were affiliated with the Pseudanabaena MIB synthase gene, demonstrating that the 2-MIB in the Han River drinking water source was produced by Pseudanabaena sp. Using a clone of the 2-MIB gene, network-based analysis and unweighted pair group method with arithmetic mean analysis were used to examine temporal and spatial variation in the 2-MIB concentration and Pseudanabaena abundance. The network analysis showed greater temporal than spatial similarity among the 2-MIB gene clones. Together, our results demonstrate that Pseudanabaena was the main producer of 2-MIB. These findings provide important information for odour management in drinking water source.