Molecular Typing of Borrelia burgdorferi.
Molecular Typing of Borrelia burgdorferi.
复制标题
伯氏疏螺旋体的分子分型。
DOI：
10.1002/9780471729259.mc12c05s34
复制
发表时间：
2014
影响因子：
--
通讯作者：
Schwartz,Ira
中科院分区：
文献类型：
--
作者：
Wang,Guiqing;Liveris,Dionysios;Mukherjee,Priyanka;Jungnick,Sabrina;Margos,Gabriele;Schwartz,Ira
伯氏疏螺旋体（Borrelia burgdorferisensu lato）是螺旋体科疏螺旋体属。螺旋体通过伊蚊科的蜱虫在宿主和宿主之间传播。感染withB。burgdorferiin引起莱姆病或莱姆博氏螺旋体病。目前，已经报道了20种莱姆病相关的疏螺旋体和20多种回归热相关的疏螺旋体。不同borrelies物种和菌株的鉴定和分化在很大程度上依赖于对其遗传特征的分析。各种分子技术已被描述为疏螺旋体的物种形成，分子流行病学和致病性研究。在本单元中，我们着重于三个基本方案，PCR - RFLP - based分型therrs - rrlAandrrfA - rrlBribosomal spacer，ospCtyping和MLST。这些方案可单独或联合用于b的表征。在蜱虫、哺乳动物宿主宿主宿主和人类临床标本中分离或直接在未培养的生物体上传播。Protoc。Microbiol 34:12C.1 12 c.31应承担的。©2014 by John Wiley & Sons, Inc。
Borrelia burgdorferisensu lato is a group of spirochetes belonging to the genusBorreliain the family of Spirochaetaceae. The spirochete is transmitted between reservoirs and hosts by ticks of the family Ixodidae. Infection withB. burgdorferiin humans causes Lyme disease or Lyme borreliosis. Currently, 20 Lyme disease‐associatedBorreliaspecies and more than 20 relapsing fever‐associatedBorreliaspecies have been described. Identification and differentiation of differentBorreliaspecies and strains is largely dependent on analyses of their genetic characteristics. A variety of molecular techniques have been described forBorreliaisolate speciation, molecular epidemiology, and pathogenicity studies. In this unit, we focus on three basic protocols, PCR‐RFLP‐based typing of therrs‐rrlAandrrfA‐rrlBribosomal spacer,ospCtyping, and MLST. These protocols can be employed alone or in combination for characterization ofB. burgdorferiisolates or directly on uncultivated organisms in ticks, mammalian host reservoirs, and human clinical specimens.Curr. Protoc. Microbiol. 34:12C.1‐12C.31. © 2014 by John Wiley & Sons, Inc.