Tear cytokine levels in the diagnosis and severity assessment of ocular chronic graft-versus-host disease(GVHD)
Tear cytokine levels in the diagnosis and severity assessment of ocular chronic graft-versus-host disease(GVHD)
复制标题
DOI：
10.1016/j.jtos.2019.12.005
复制
发表时间：
2020-04-01
期刊：
影响因子：
6.4
通讯作者：
Hong, Jing
中科院分区：
文献类型：
--
作者：
Hu, Bohao;Qiu, Yuan;Hong, Jing
目的：分析眼部慢性移植物抗宿主病（GVHD）患者泪液中细胞因子水平，并检测其与不同眼表参数的一致性。选择23例眼部慢性GVHD患者，评估泪液细胞因子水平和眼表参数方法：选择16例无全身免疫性疾病的干眼症（DED）患者作为对照组，进行TBUT、CFS、OSDI、Schirmer试验检测。结果：oGVHD患者眼内ICAM-1、IL-1 β、IL-6和IL-8水平均高于DED患者眼内[P < 0.001]; oGVHD患者眼内IL-7和EGF水平低于DED患者眼内IL-7和EGF水平[P < 0.0001]。此外，IL-6和IL-8水平与角膜荧光素染色（CFS）显示更强的相关性IL-6和ICAM-1水平与荧光素泪膜破裂时间（TBUT）最为一致（P < 0.05）。结论：我们的研究表明某些泪液细胞因子，包括ICAM-1，IL-1 β，IL-6，IL-8，IL-7和EGF，作为有前途的新的可能的慢性oGVHD的诊断标志物和慢性眼部GVHD严重性的标准。由于泪液取样是无创的和简单的，这种方法预计将是一个压倒性的适用于筛查和诊断慢性GVHD。
Purpose: To analyse the tear cytokine levels of patients diagnosed with ocular chronic graft-versus-host disease (GVHD)and examine the consistency of these levels with different ocular surface parameters.Methods: 23 ocular chronic GVHD patients were selected for the assessment of tear cytokine levels and ocular surface parameters (TBUT, CFS, OSDI, and Schirmer's test), and 16 dry eye disease(DED)patients without systemic immune disease were selected as the control group. The 23 cytokines were measured using microsphere-based immunoassay analysis and their consistency with different ocular surface parameters was studied.Results: ICAM-1, IL-1 beta, IL-6, and IL-8 showed elevated levels in the eyes of oGVHD patients compared to DED [P < 0.001]. IL-7 and EGF showed lower levels in the eyes of patients with oGVHD than in those of patients with DED [P < 0.0001]. Furthermore, the levels of IL-6 and IL-8 showed a stronger correlation with corneal fluorescein staining (CFS) (P < 0.05), and the levels of IL-6 and ICAM-1 were most consistent with fluorescein tear film break-up time (TBUT) (P < 0.05).Conclusions: Our study demonstrated certain tear cytokines, including ICAM-1, IL-1 beta, IL-6, IL-8 IL-7 and EGF, as promising new possible diagnostic markers of chronic oGVHD and criteria for chronic ocular GVHD severity. Because tear sampling is noninvasive and simple, this method is expected to be an overwhelming applicable for the screening and diagnosis of chronic GVHD.