Effects of insulin-like growth factor-1 on endoplasmic reticulum stress and autophagy in rat gastric smooth muscle cells cultured at different glucose concentrations in vitro
Effects of insulin-like growth factor-1 on endoplasmic reticulum stress and autophagy in rat gastric smooth muscle cells cultured at different glucose concentrations in vitro
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胰岛素样生长因子1对不同葡萄糖浓度体外培养的大鼠胃平滑肌细胞内质网应激和自噬的影响
DOI：
10.1007/s11010-018-3388-7
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发表时间：
2018-07
影响因子：
4.3
通讯作者：
Jin Zheng
中科院分区：
文献类型：
--
作者：
Fang Xue sen;Zhang Mo han;Guo Jun yu;Jin Zheng
The purpose of the study was to observe changes in endoplasmic reticulum stress (ERS)- and autophagy-related proteins in gastric smooth muscle tissues of diabetic rats with gastroparesis, investigate the effect of insulin-like growth factor 1 (IGF-1) on ERS and autophagy in rat gastric smooth muscle cells cultured under different glucose concentrations, and explore the influence of IGF-1 on development of diabetic gastroparesis (DGP). After establishing a rat model of DGP, rats were divided into normal control (NC) and 6-week diabetic model (DM6W) groups. Expression of ERS-related and autophagy-related proteins was detected by western blot analysis and immunofluorescence assay in rat gastric smooth muscle tissue and in vitro-cultured rat gastric smooth muscle cells exposed to different glucose concentrations and treatment with IGF-1 for 24 or 48 h. Changes in glucose-regulated-protein-78 (GRP78), growth arrest and DNA damage-inducible gene 153 (CHOP), and microtubule-associated protein 1A/1B light chain 3B (LC3) expression levels were detected by western blot analysis, and GRP78 and LC3 expression were examined by confocal laser-scanning microscopy. In vivo expression levels of GRP78, CHOP, and LC3 were significantly higher in the DM6W group compared with the NC group (p< 0.001). Twenty-four hours after cells were cultured at different glucose concentrations in vitro, expression of GRP78, CHOP, and LC3II/I was significantly higher in the high glucose-treated group compared with the normal glucose group (p< 0.05). After IGF-1 intervention, CHOP and GRP78 expression were significantly higher in the normal glucose + IGF-1 group compared with the normal glucose group (p< 0.01), while no significant difference was found between high glucose and high glucose + IGF-1 groups. LC3II/I expression was significantly lower in the normal glucose + IGF-1 group compared with the normal glucose group, and was significantly lower in the high glucose and high glucose + IGF-1 groups (p< 0.05). After 48 h of culture, CHOP expression was significantly higher and LC3II/I expression was significantly lower in the high glucose group compared with the normal glucose group (p< 0.05), but no significant change in GRP78 expression was observed between these two groups. After IGF-1 intervention, there was no difference in CHOP or GRP78 expression between normal glucose + IGF-1 and normal glucose groups. However, CHOP and GRP78 expression were significantly lower in the high glucose + IGF-1 group compared with the high glucose group (p< 0.05). There was no significant difference in LC3II/I expression between normal glucose + IGF-1 and normal glucose groups, or high glucose + IGF-1 and high glucose groups. Results of confocal laser-scanning microscopy showed significantly lower expression of LC3II/I in the high glucose + IGF-1 group compared with the high glucose group (p< 0.05). ERS and autophagy were involved in the occurrence of DGP. IGF-1 exerted an inhibitory effect on ERS in rat gastric smooth muscle cells cultured under high glucose conditions, and this inhibitory effect increased with time. IGF-1 inhibited the level of autophagy in rat gastric smooth muscle cells cultured under high glucose conditions at early stages, which may be achieved through inhibition of ERS.
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影响因子：
5.6
作者：
Pfaffenbach, Kyle T.;Pong, Michelle;Morgan, Todd E.;Wang, Hongjun;Ott, Kate;Zhou, Beiyun;Longo, Valter D.;Lee, Amy S.
通讯作者：
Lee, Amy S.
高脂肪含量和饱和脂肪酸棕榈酸酯抑制软骨细胞中的IGF-1功能。
影响因子：
7
作者：
Nazli SA;Loeser RF;Chubinskaya S;Willey JS;Yammani RR
通讯作者：
Yammani RR
自噬通过保护高血糖诱导的荚膜细胞损伤，减轻糖尿病肾小球损伤。
影响因子：
3.7
作者：
Fang L;Zhou Y;Cao H;Wen P;Jiang L;He W;Dai C;Yang J
通讯作者：
Yang J
影响因子：
5.1
作者：
Roohbakhsh, Ali;Shirani, Kobra;Karimi, Gholamreza
通讯作者：
Karimi, Gholamreza
影响因子：
--
作者：
Yang Zhou;Wei Wu
通讯作者：
Yang Zhou;Wei Wu