Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant Fasciola hepatica
Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant Fasciola hepatica
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DOI：
10.1017/s0031182013000528
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发表时间：
2013-10-01
期刊：
影响因子：
2.4
通讯作者：
Williams, D. J. L.
中科院分区：
文献类型：
--
作者：
Hodgkinson, J.;Cwiklinski, K.;Williams, D. J. L.
尽管多年来对肝片吸虫对三氯苯达唑(TCBZ)的耐药性进行了研究，但其遗传机制仍不清楚。使用体内实验和体外实验相结合的方法对多种三氯苯达唑敏感和耐药菌株进行了广泛的分析，但很少有基因被实验证明与田间耐药表型有关。在这篇综述中，我们从迄今为止所采用的方法总结了目前对TCBZ耐药性的理解。我们报道了目前肝片吸虫的基因组和遗传资源，这些资源可用于促进未来针对这种寄生虫的新的功能基因组学和遗传学实验。最后，我们描述了我们自己的无偏见的方法来定位与肝片吸虫对TCBZ抗性有关的主要遗传位点。
Despite years of investigation into triclabendazole (TCBZ) resistance in Fasciola hepatica, the genetic mechanisms responsible remain unknown. Extensive analysis of multiple triclabendazole-susceptible and -resistant isolates using a combination of experimental in vivo and in vitro approaches has been carried out, yet few, if any, genes have been demonstrated experimentally to be associated with resistance phenotypes in the field. In this review we summarize the current understanding of TCBZ resistance from the approaches employed to date. We report the current genomic and genetic resources for F. hepatica that are available to facilitate novel functional genomics and genetic experiments for this parasite in the future. Finally, we describe our own non-biased approach to mapping the major genetic loci involved in conferring TCBZ resistance in F. hepatica.