DNA nanotechnology assisted nanopore-based analysis
DNA nanotechnology assisted nanopore-based analysis
复制标题
DNA 纳米技术辅助基于纳米孔的分析
DOI：
10.1093/nar/gkaa095
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发表时间：
2020-04-06
影响因子：
14.9
通讯作者：
Zhang, Cheng
中科院分区：
文献类型：
--
作者：
Ding, Taoli;Yang, Jing;Zhang, Cheng
摘要 纳米孔技术是一种很有前景的免标记检测方法。然而，其在测序、临床诊断和超灵敏单分子检测方面的进一步应用还存在挑战。尽管如此，DNA 纳米技术的发展为当前阻碍纳米孔传感技术的障碍提供了可能的解决方案。在这篇综述中，我们总结了最近致力于开发与 DNA 纳米技术相关的纳米孔方法的相关研究。例如，DNA载体可以在预先设计的位点捕获特定目标，并以适当的速度护送它们离开纳米孔，从而大大提高特定目标分子的检测能力和分辨率。此外，可以构建DNA折纸结构来满足各种设计规范和一锅组装反应，从而充当功能性纳米孔。此外，基于DNA链置换，纳米孔还可用于表征DNA计算的输出并开发可编程智能诊断纳米器件。总之，基于 DNA 组装的纳米孔研究可以为通过单生物分子分析实现有影响力的生物检测和诊断平台铺平道路。
Abstract Nanopore technology is a promising label-free detection method. However, challenges exist for its further application in sequencing, clinical diagnostics and ultra-sensitive single molecule detection. The development of DNA nanotechnology nonetheless provides possible solutions to current obstacles hindering nanopore sensing technologies. In this review, we summarize recent relevant research contributing to efforts for developing nanopore methods associated with DNA nanotechnology. For example, DNA carriers can capture specific targets at pre-designed sites and escort them from nanopores at suitable speeds, thereby greatly enhancing capability and resolution for the detection of specific target molecules. In addition, DNA origami structures can be constructed to fulfill various design specifications and one-pot assembly reactions, thus serving as functional nanopores. Moreover, based on DNA strand displacement, nanopores can also be utilized to characterize the outputs of DNA computing and to develop programmable smart diagnostic nanodevices. In summary, DNA assembly-based nanopore research can pave the way for the realization of impactful biological detection and diagnostic platforms via single-biomolecule analysis.