Monoclonal antibodies to cell surface antigens involved in sex pheromone induced mating in Streptococcus faecalis.
Monoclonal antibodies to cell surface antigens involved in sex pheromone induced mating in Streptococcus faecalis.
复制标题
针对参与粪链球菌性信息素诱导交配的细胞表面抗原的单克隆抗体。
DOI：
10.1099/00221287-132-4-857
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Dunny,G
中科院分区：
文献类型：
--
作者：
Tortorello,M;Adsit,J;Krug,D;Antczak,D;Dunny,G
构建了粪链球菌表面抗原单克隆抗体杂交瘤细胞株。分离的一些抗体直接针对参与信息素诱导交配的表面成分。本文介绍了单克隆抗体在免疫沉淀和Western blotting检测中鉴定抗原性相关表面成分的应用，它们在信息素反应分析中的应用，以及它们在交配实验中作为功能抑制剂的应用。
Hybridoma cell lines producing monoclonal antibodies toStreptococcus faecaliscell surface antigens were constructed. Some of the antibodies isolated were directed against surface components involved in pheromone-induced mating. This paper describes the use of the monoclonal antibodies to identify antigenically related surface components detected by immunoprecipitation and Western blotting, their use in pheromone response assays, and their use as functional inhibitors in mating experiments.