Gene expression analysis of autofluorescence margins in leukoplakia and oral carcinoma: A pilot study
Gene expression analysis of autofluorescence margins in leukoplakia and oral carcinoma: A pilot study
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DOI：
10.1111/odi.13525
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发表时间：
2020-08-03
期刊：
影响因子：
3.8
通讯作者：
Giudice, Amerigo
中科院分区：
文献类型：
--
作者：
Biamonte, Flavia;Buffone, Caterina;Giudice, Amerigo
目的自体荧光被认为是早期发现口腔粘膜病变的有用技术。然而，其区分肿瘤边缘的有效性仍在争论中。本初步研究的目的是证实白斑和口腔癌中与白色光和自体荧光（VELscope（TM））可视化边缘相比，病变中心存在分子分歧。材料与方法比较3例口腔白斑和4例口腔癌患者病变中心到白色光和VELscope（TM）边缘的分子差异。通过定制的TaqMan阵列进行45个选定基因的表达谱分析。基因本体用于生物途径分析。结果无论病理类型如何，病变中心与白色光和VELscope（TM）边缘的分子差异最大。VELscope（TM）和白色光边缘在口腔癌样品中也是分子上不同的。事实上，白色光边缘保留了在病变中心观察到的分子异常，因此表明存在“部分转化”的细胞群。结论尽管患者数量有限，但我们的数据证实了自体荧光与常规口腔检查相结合在白斑和口腔癌活检过程中识别手术切缘的益处。
Objectives Autofluorescence is considered a useful technique in the early detection of oral mucosal alterations. However, its efficacy to discriminate tumor margins is still under debate. The purpose of this pilot study was to confirm the existence of molecular divergence from the center of a lesion compared to white light and autofluorescence (VELscope(TM)) visualized margins in leukoplakia and oral carcinoma. Materials and methods Molecular divergence from the center of the lesion to white light and VELscope(TM)defined margins was compared in patients with leukoplakia (n = 3) and oral carcinoma (n = 4). Expression profiling of 45 selected genes was performed through custom-made TaqMan arrays. Gene Ontology was used for biological pathway analysis. Results Irrespective of pathology, the greatest molecular divergence existed between the center of the lesion and both white light and VELscope(TM)margins. VELscope(TM)and white light margins were also molecularly distinct in oral carcinoma samples. Indeed, the white light margin retained molecular abnormalities observed in the center of the lesion thus suggesting the existence of a "partially transformed" cell population. Conclusion Despite the limited low number of patients, our data confirm the benefit of combining autofluorescence with conventional oral examination in identifying surgical margins during biopsy procedures for leukoplakia and oral carcinoma.