Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.)
Transcriptome profiling reveals the candidate genes associated with aroma metabolites and emission of pear (Pyrus ussuriensis cv.)
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转录组分析揭示了与梨（Pyrus ussuriensis cv.）香气代谢物和散发相关的候选基因
DOI：
10.1016/j.scienta.2016.04.019
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发表时间：
2016
影响因子：
4.3
通讯作者：
Zhang Shaoling
中科院分区：
文献类型：
--
作者：
Wei Shuwei;Tao Shutian;Qin Gaihua;Wang Shaomin;Tao Jihan;Wu Jun;Wu Juyou;Zhang Shaoling
香气是影响梨果实品质的重要因素。然而，香气代谢物的发展及其排放的分子基础知之甚少。未成熟的'南果梨'果实香气较差，而成熟果实香气浓郁，钙处理可以增强果实香气，为转录组分析提供了良好的材料。在定位的读段总数（11.4-13.6百万）中，平均69.81%可以定位到梨基因组。根据GO数据库和KEGG数据库，将10，776个差香和浓香差异表达基因分别归为36个功能组和37条通路。在更芳香的果实中更高表达的基因包括一些来自脂肪酸途径的基因，如FAD、ADH、PDC、LOX和脂肪酶基因。在梨果实中发现了一些与香气合成高度相关的转录因子。阿坝可能在钙引发的南果梨香气提升中起重要作用。此外，候选基因进行了验证与定量实时PCR。
Aroma is an important factor affecting pear fruit quality. However, the molecular basis for the development of aroma metabolites and their emission is poorly understood. Unripe ‘Nanguoli’ pear fruit has poor aroma, while the ripe fruit has an intense aroma, and calcium treatment can enhance the aroma, providing good material for transcriptome analysis.Digital gene expression (DGE) analysis of ‘Nanguoli’ fruit was performed at three important developmental stages, with and without calcium treatment, six libraries in total. Of the total number of mapped reads (11.4–13.6 million), an average of 69.81% could be mapped to the pear genome. A total of 10,776 differentially expressed genes between the poor aroma and intense aroma were categorized into 36 functional groups and 37 pathways according to GO and KEGG databases, respectively. Genes more highly expressed in the more aromatic fruit include some from the fatty acid pathway such asFAD, ADH, PDC, LOX,and lipase genes. Some transcription factors were identified with high correlation to aroma biosynthesis in pear. Also, ABA may play an important role in the calcium-triggered improvement of ‘Nanguoli’ aroma. In addition, candidate genes were verified with quantitative real-time PCR.