Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus
Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus
复制标题
DOI：
10.1099/ijs.0.005488-0
复制
发表时间：
2009-09-01
影响因子：
2.8
通讯作者：
Roux, Veronique
中科院分区：
文献类型：
--
作者：
Glazunova, Olga O.;Raoult, Didier;Roux, Veronique
使用 16S rRNA 基因（1468-1478 bp）、rpoB（编码 RNA 聚合酶的 β 亚基）（659-680 bp）、sodA（编码锰依赖性超氧化物歧化酶）（435-462 bp）、groEL（编码 60 kDa）的部分序列比较，对链球菌属成员进行系统发育分析和物种鉴定。热休克蛋白（757 bp）和gyrB，编码DNA旋转酶B亚基（458-461 bp）。该研究首次涵盖了链球菌属内的大多数物种（65 个菌株，代表 58 个物种和 9 个亚种）。从 rpoB、sodA、gyrB 和 groEL 序列比较推断的系统发育比从 16S rRNA 基因序列比较推断的系统发育更具辨别力，并且显示出共同的簇。 16S rRNA 基因、rpoB、sodA、gyrB 和 groEL 的最小种间差异分别为 0.3%、2.7%、0%、2.5% 和 3.4%。一般来说，groEL 部分基因序列比较是鉴定物种和亚种以及系统发育分析的最佳工具。
Phylogenetic analysis and species identification of members of the genus Streptococcus were carried out using partial sequence comparison of the 16S rRNA gene (1468-1478 bp), rpoB, encoding the beta subunit of RNA polymerase (659-680 bp), sodA, encoding the manganese-dependent superoxide dismutase (435-462 bp), groEL, encoding the 60 kDa heat-shock protein (757 bp), and gyrB, encoding the B subunit of DNA gyrase (458-461 bp). For the first time, most species within the genus Streptococcus were represented in the study (65 strains, representing 58 species and nine subspecies). Phylogenies inferred from rpoB, sodA, gyrB and groEL sequence comparisons were more discriminative than those inferred from 16S rRNA gene sequence comparison, and showed common clusters. The minimal interspecies divergence was 0.3, 2.7, 0, 2.5 and 3.4% for the 16S rRNA gene, rpoB, sodA, gyrB and groEL, respectively. In general, groEL partial gene sequence comparison represented the best tool for identifying species and subspecies and for phylogenetic analysis.