Neural correlates of IgE-mediated allergy
Neural correlates of IgE-mediated allergy
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DOI：
10.1196/annals.1366.028
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发表时间：
2006-01-01
期刊：
影响因子：
--
通讯作者：
Palermo-Neto, Joao
中科院分区：
文献类型：
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作者：
Costa-Pinto, Frederico Azevedo;Basso, Alexandre Salgado;Palermo-Neto, Joao
尽管许多作者认为过敏反应和行为变化之间存在直接的相互作用，但支持证据一直难以捉摸。在这一系列研究中，我们发现，口服或鼻腔注射卵清蛋白(OVA)后，过敏小鼠下丘脑室旁核(PVN)和杏仁中央核(CEA)的Fos表达增加。食物过敏的小鼠表现出更高的焦虑水平和血清皮质酮水平的升高，过敏激活的神经元在PVN和CEA中表达促肾上腺皮质激素释放因子(CRF)。对卵子过敏的小鼠会对含有抗原的溶液产生厌恶，也会避免以前雾化的OVA产生的黑暗隔间。大脑Fos表达和行为数据的结果似乎与适应性反应一致。通过抗体耗竭或口服耐受性的发展去除IgE排除了这里所分析的所有反应。新生辣椒素破坏C敏感纤维抑制了PVN的激活，但不抑制CEA的激活，并降低了对食物的厌恶程度。肥大细胞稳定剂Cromolyn完全阻断了PVN和CEA中Fos的表达，并阻止了与雾化OVA相关的对暗室的厌恶。使用在鼻腔OVA激发后没有形成重要炎症浸润物的小鼠，我们发现激发部位不需要炎性细胞来触发小鼠实验性哮喘的神经或行为相关。总之，我们已经为理解过敏反应中的神经免疫相互作用奠定了坚实的基础，这可能有助于理解与过敏相关的心理障碍。
Although many authors have considered a direct interaction between allergic reactions and behavioral changes, supporting evidence has been elusive. In this series of studies we show that after oral or nasal ovalbumin (OVA) challenge, allergic mice present increased Fos expression in the paraventricular nucleus of the hypothalamus (PVN) and in the central nucleus of the amygdala (CeA). Mice with food allergy display higher levels of anxiety and increased serum corticosterone levels, and allergy-activated neurons express corticotropin-releasing factor (CRF) in the PVN and CeA. OVA-allergic mice develop aversion to an antigen-containing solution, and also avoid a dark compartment previously associated with nebulized OVA. Results on brain Fos expression and behavioral data seem compatible with adaptive responses. Removal of IgE by either antibody depletion or the development of oral tolerance precluded all responses analyzed here. C-sensitive fiber destruction by neonatal capsaicin inhibited the activation in the PVN, but not in the CeA, and decreased the magnitude of food aversion. Cromolyn, a mast cell stabilizer, completely blocked Fos expression in the PVN and CeA, and precluded the development of aversion to the dark compartment associated with nebulized OVA. Employing mice that do not develop an important inflammatory infiltrate following nasal OVA challenge, we found that inflammatory cells are not required at the site of challenge in order to trigger neural or behavioral correlates of murine experimental asthma. Altogether, we have built a solid foundation for understanding neuroimmune interactions during allergic responses that may contribute to the comprehension of psychological disorders associated with allergy.