Effect of glutaraldehyde on renal tubular function
Effect of glutaraldehyde on renal tubular function
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戊二醛对肾小管功能的影响
DOI：
10.1007/bf00585073
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
M. Imai
中科院分区：
文献类型：
--
作者：
Y. Kondo;M. Imai
为了进一步研究Henle’s loop （TAL）薄升肢的Cl -转运，我们观察了戊二醛对体外灌流的仓鼠TAL中Na+和Cl -转运的影响。我们发现，当0.1 mol/l戊二醛加入管腔或液中时，NaCl扩散电位会迅速逆转。这主要是由于Cl -通透性（10 - 7 cm2 s - 1）从93.51±8.39抑制到14.89±3.91 （P<0.01,n=9）。相比之下，Na+渗透率从34.18±3.27到26.56±2.74变化不大（P<0.01,n=6）。戊二醛处理消除了TAL对卤素的选择性，这是由离子取代时看到的电压偏转所决定的。Cl−、Br−、I−和SCN−相对于Na+的渗透率分别从3.16±0.20、3.22±0.19、2.97±0.26和4.36±0.36变化为0.38±0.07、0.35±0.06、0.36±0.07和0.58±0.05。在10−5 ~ 10−1 m范围内，戊二醛对NaCl扩散电位的影响呈剂量依赖性，在浓度低于10−3 m时，这种影响是可逆的。这些观察结果构成了Cl−跨TAL输运机制不同于Na+输运机制的额外证据。戊二醛可能通过交联卤素运输必需蛋白质的氨基酸残基来抑制TAL中Cl−的运输。
In order to further characterize Cl− transport of the thin ascending limb of Henle's loop (TAL), we observed the effects of glutaraldehyde on Na+ and Cl− transport in hamster TAL perfused in vitro. We found that 0.1 mol/l glutaraldehyde added either to the lumen or to the bath caused a rapid irreversible reversal of the NaCl diffusion potential. This was mainly accounted for by an inhibition of Cl− permeability (10−7 cm2 s−1) from 93.51±8.39 to 14.89±3.91 (P<0.01,n=9). By contrast, Na+ permeability changed little from 34.18±3.27 to 26.56±2.74 (P<0.01,n=6). Glutaraldehyde treatment abolished the halogen-permselectivity of the TAL as determined by the voltage deflection seen upon ionic substitution. Permeabilities for Cl−, Br−, I−, and SCN− relative to Na+ were changed from 3.16±0.20, 3.22±0.19, 2.97±0.26 and 4.36±0.36 to 0.38±0.07, 0.35±0.06, 0.36±0.07 and 0.58±0.05, respectively. The effect of glutaraldehyde on the NaCl diffusion potential was dose-dependent in the range from 10−5 to 10−1 M. The effect was reversible at concentrations lower than 10−3 M. Glutaraldehyde did not affect the NaCl diffusion potential of the long-loop descending limb. These observations constitute additional evidence that the mechanism of Cl− transport across the TAL is different from that of Na+ transport. Glutaraldehyde might inhibit Cl− transport in the TAL by cross-linking amino acid residues of the proteins essential for halogen transport across this segment.