Ethanol consumption enhances periodontal inflammatory markers in rats
Ethanol consumption enhances periodontal inflammatory markers in rats
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DOI：
10.1016/j.archoralbio.2012.02.008
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发表时间：
2012-09-01
影响因子：
3
通讯作者：
Fernandez-Solari, Javier
中科院分区：
文献类型：
--
作者：
Maia Dantas, Aline;Ester Mohn, Claudia;Fernandez-Solari, Javier
目的：探讨乙醇对大鼠牙周病的短期疗效。设计：大鼠每天灌胃2次乙醇2g/kg或水2次。第5天，用结扎法将半数大鼠磨牙结扎，造成牙周炎。7天后取出牙龈组织，进行炎症标志物检测。最后，采用组织形态计量学技术，观察大鼠半下颌骨的骨丢失情况。结果：实验性牙周炎组大鼠牙周组织诱导型一氧化氮合酶(INOSmRNA)表达显著增加(p<；0.001)，诱导型一氧化氮合酶(INOS)活性显著升高(p<；0.001)，短期乙醇染毒可显著提高iNOS活性(p<；0.05)，且对牙周炎引起的iNOSmRNA表达具有相加作用(p<；0.01)。短期给予乙醇也增强了牙周炎对IL-1β基因表达的刺激作用(半定量和实时定量分别为P<；0.01和P<；0.001)和对牙周韧带高度的刺激作用(P<；0.05)。然而，与假手术组相比，结扎诱导的牙周炎大鼠的前列腺素E-2含量增加(p<；0.05)，牙槽骨体积减小(p<；0.05)。结论：在健康大鼠中，酒精摄入可增加炎症标记物的表达，并在短期内增加炎症标记物的表达，因此可能是牙周病的危险指标。然而，还需要规模纵向调查和更多的病例对照研究来证实这一说法。(C)2012爱思唯尔有限公司。保留所有权利。
Objective: The aim of this study was to assess the short term effect of ethanol administration on periodontal disease in rats.Design: Rats received either ethanol 2 g/kg or water by gastric gavage twice a day. On the fifth day ligatures were tied around the molars of half of the rats to induce periodontitis. After 7 days gingival tissue was removed and assayed for inflammatory markers. Finally, hemi-mandibles were extracted to evaluate bone loss by histomorphometrical techniques. Results: The experimental periodontitis increased significantly the mRNA expression (p < 0.001) and activity (p < 0.001) of inducible nitric oxide synthase (iNOS) in the gingival tissue, whilst short time ethanol administration increased iNOS activity (p < 0.05) and produced an additive effect on iNOS mRNA expression augmented by periodontitis (p < 0.01). The short time ethanol administration also potentiated the periodontitis stimulatory effect on the mRNA expression of interleukin (IL)-1 beta (p < 0.01 and p < 0.001, in semi-quantitative and real time PCR, respectively) and on the height of periodontal ligament (p < 0.05). However, the ligature-induced periodontitis, but not ethanol administration, increased the prostaglandin E-2 content (p < 0.05) and, diminished the alveolar bone volume (p < 0.05), as compared to sham rats.Conclusion: The present results suggest that ethanol consumption could represent a risk indicator for periodontal disease since augments the expression of inflammatory markers, in healthy rats, and increases them, at short term, during the illness. However, scale longitudinal investigation and more case-control studies are needed to confirm this statement. (C) 2012 Elsevier Ltd. All rights reserved.