Three-Dimensional Human Tissue Models of Wounded Skin
Three-Dimensional Human Tissue Models of Wounded Skin
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DOI：
10.1007/978-1-60761-380-0_24
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Shamis, Yulia
中科院分区：
文献类型：
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作者：
Egles, Christophe;Garlick, Jonathan A.;Shamis, Yulia
人皮肤等同物（HSE）是体外组织，其中丘状分化的复层鳞状上皮在含有人真皮成纤维细胞的I型胶原凝胶上的气-液界面处生长。HSEs现在提供了实验性人体组织模型来研究创伤后直接上皮再生和上皮-间充质串扰的因素。本章描述了从人角质形成细胞和成纤维细胞中制备HSEs，以及如何对HSEs进行修饰以表征伤口修复过程中人上皮细胞的反应。首先概述的方案描述了用于产生非常接近人类皮肤的结构特征、分化和生长的人类组织的技术。随后将描述一个协议，使HSE适应监测他们的反应后受伤。这些工程化的人体组织提供了强有力的工具来研究模拟人体皮肤和上皮组织愈合的组织中的生物过程。
Human skin equivalents (HSEs) arc in vitro tissues in which a hilly differentiated, stratified squamous epithelium is grown at an air-liquid interface on a Type I collagen gel harboring human dermal fibroblasts. HSEs now provide experimental human tissue models to study factors that direct reepithelialization and epithelial-mesenchymal cross-talk following wounding. This chapter describes the fabrication of HSEs from human keratinocytes and fibroblasts and how HSEs can be modified to characterize the response of the human epithelium during wound repair. The protocols outlined first describe techniques for the generation of human tissues that closely approximate the architectural features, differentiation, and growth of human skin. This will be followed by a description of a protocol that enables HSEs to be adapted to monitor their response following wounding. These engineered human tissues provide powerful tools to study biological process in tissues that mimic the healing of human skin and of the epithelial tissue.