Electrosteric Enhanced Stability of Functional Sub-10 nm Cerium and Iron Oxide Particles in Cell Culture Medium
Electrosteric Enhanced Stability of Functional Sub-10 nm Cerium and Iron Oxide Particles in Cell Culture Medium
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DOI：
10.1021/la900833v
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发表时间：
2009-08-18
期刊：
影响因子：
3.9
通讯作者：
Berret, J. -F.
中科院分区：
文献类型：
--
作者：
Chanteau, B.;Fresnais, J.;Berret, J. -F.
纳米颗粒在生物学中的应用要求纳米颗粒在含有高浓度蛋白质和盐的溶液以及细胞培养基中保持稳定。在这项工作中，我们开发了用于涂覆亚 10 nm 纳米颗粒的简单方案，并评估了分散体在各种环境中的胶体稳定性。配体（柠檬酸）、低聚物[膦酸酯封端的聚（环氧乙烷）]和聚合物[聚丙烯酸]被用作氧化铈（CeO2）和氧化铁（γ-Fe2O3）纳米颗粒的纳米厚度吸附层。有机官能团通过物理（静电）力吸附在颗粒表面。通过静态和动态光散射在高离子强度和细胞 CUI Lure 介质中进行稳定性测定。在所检查的三种涂料中，我们发现只有聚丙烯酸能够长期（超过几周）完全保持分散稳定性。稳定性的提高可以通过链在颗粒表面上的多点附着以及吸附层介导的电空间相互作用来解释。这些结果表明，带阴离子电荷的聚合物是传统涂层剂的有效替代品。
Applications of nanoparticles in biology require that the nanoparticles remain stable in solutions containing high concentrations of proteins and salts, as well as in cell culture media. In this work, we developed simple protocols for the coating of sub-10 nm nanoparticles and evaluated the colloidal stability of dispersions in various environments. Ligands (citric acid), oligomers [phosphonate-terminated poly(ethylene oxide)], and polymers [poly(acrylic acid)] were used as nanometer-thick adlayers for cerium (CeO2) and iron (gamma-Fe2O3) oxide nanoparticles. The organic functionalities were adsorbed on the particle surfaces via physical (electrostatic) forces. Stability assays at high ionic strengths and in cell CUI Lure media were performed by static and dynamic light scattering. Of the three coatings examined, we found that only poly(acrylic acid) fully preserved the dispersion stability over the long term (longer than weeks). The improved stability was explained by till multipoint attachments of the chains onto the particle surface and by the adlayer-mediated electrosteric interactions. These results suggest that anionically charged polymers represent an effective alternative to conventional coating agents.