Characterization of the gene for mp20: a Drosophila muscle protein that is not found in asynchronous oscillatory flight muscle.
Characterization of the gene for mp20: a Drosophila muscle protein that is not found in asynchronous oscillatory flight muscle.
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DOI：
10.1083/jcb.108.2.521
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发表时间：
1989-02
期刊：
影响因子：
--
通讯作者：
Pardue ML
中科院分区：
文献类型：
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作者：
Ayme-Southgate A;Lasko P;French C;Pardue ML
用肌管原代培养物中的RNA进行差异筛选，获得了编码肌肉特异性蛋白的果蝇黑腹果蝇基因。该基因编码一种20kD的蛋白质，肌肉蛋白20(Mp20)，在异步振荡飞行的肌肉中没有检测到，但在大多数(如果不是全部)其他肌肉(同步肌肉)中发现。该蛋白质的序列由DNA推导而来，包含12个氨基酸的两个区域，与其他蛋白质的高亲和力钙结合部位有很大的相似性。这种蛋白质很容易从收缩器官中提取出来，因此似乎不是一个紧密结合的结构成分。该基因(位于多线区49F9-13)在黑腹隐翅虫基因组中是独一无二的，并产生两个转录本，分别为1.0和0.9kb。这两个转录本的水平在发育过程中受到不同的调控，但两个转录本的编码区是相同的。
A Drosophila melanogaster gene encoding a muscle specific protein was isolated by differential screening with RNA from primary cultures of myotubes. The gene encodes a 20-kD protein, muscle protein 20 (mp20), that is not detected in the asynchronous oscillatory flight muscles, but is found in most, if not all, other muscles (the synchronous muscles). The sequence of the protein, deduced from the DNA, contains two regions of 12 amino acids with significant similarity to high- affinity calcium-binding sites of other proteins. This protein is easily extracted from the contractile apparatus and thus does not seem to be a tightly bound structural component. The gene (located in polytene region 49F 9-13) is unique in the D. melanogaster genome and yields two transcripts, 1.0 and 0.9 kb long. The levels of the two transcripts are regulated differently during development, yet the coding regions of the two transcripts are identical.