Natural variation at a single gene generates sexual antagonism across fitness components in Drosophila
Natural variation at a single gene generates sexual antagonism across fitness components in Drosophila
复制标题
DOI：
10.1016/j.cub.2022.05.038
复制
发表时间：
2022-06
期刊：
影响因子：
9.2
通讯作者：
B. Rusuwa;Henry Chung;S. Allen;F. Frentiu;S. Chenoweth
中科院分区：
文献类型：
--
作者：
B. Rusuwa;Henry Chung;S. Allen;F. Frentiu;S. Chenoweth
在雄性和雌性种群中具有相互冲突的适应性效应的突变在有性种群中积累，降低了它们的适应能力。1,2尽管定量遗传学研究表明性拮抗多态性是常见的，但它们的分子基础和群体遗传特性仍然知之甚少。6,7在这里，我们在果蝇中展示了单个基因的自然变异是如何通过对表皮碳氢化合物（CHC）性状的表型影响产生性拮抗的，这些性状既是交配信号，也是跨纬度梯度抵御非生物胁迫的保护因子。热带果蝇种群具有多态CHCs，通过对这两种适应性相关功能的相反但性别限制的作用产生性拮抗。我们将这种多态性分析为单个脂肪酰基辅酶a还原酶基因DsFAR2-B，该基因在合成CHCs的卵泡细胞中表达。rnai介导的dsfar2 - bortholog d .黑素胃肥大细胞的破坏影响CHCs的方式与看到的d . serrata相似。种群基因组分析表明，平衡选择可能在野生dsfar2 - blocus中起作用。我们的研究提供了对自然界性别拮抗的遗传基础的见解，并将表型上性别差异的拮抗选择与维持分子变异的基因型平衡选择联系起来。
Mutations with conflicting fitness effects in males and females accumulate in sexual populations, reducing their adaptive capacity.1,2Although quantitative genetic studies indicate that sexually antagonistic polymorphisms are common,3–5their molecular basis and population genetic properties remain poorly understood.6,7Here, we show in fruit flies how natural variation at a single gene generates sexual antagonism through phenotypic effects on cuticular hydrocarbon (CHC) traits that function as both mate signals and protectors against abiotic stress8across a latitudinal gradient. Tropical populations ofDrosophila serratahave polymorphic CHCs producing sexual antagonism through opposing but sex-limited effects on these two fitness-related functions. We dissected this polymorphism to a single fatty-acyl CoA reductase gene,DsFAR2-B, that is expressed in oenocyte cells where CHCs are synthesized. RNAi-mediated disruption of theDsFAR2-Bortholog inD. melanogasteroenocytes affected CHCs in a similar way to that seen inD. serrata. Population genomic analysis revealed that balancing selection likely operates at theDsFAR2-Blocus in the wild. Our study provides insights into the genetic basis of sexual antagonism in nature and connects sexually varying antagonistic selection on phenotypes with balancing selection on genotypes that maintains molecular variation.