Excision repair of O6-methylguanine synthesized at the rat H-ras N-methyl-N-nitrosourea activation site and introduced into Escherichia coli.
Excision repair of O6-methylguanine synthesized at the rat H-ras N-methyl-N-nitrosourea activation site and introduced into Escherichia coli.
复制标题
切除修复在大鼠 H-ras N-甲基-N-亚硝基脲激活位点合成并引入大肠杆菌的 O6-甲基鸟嘌呤。
DOI：
10.1093/carcin/10.2.373
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发表时间：
1989
期刊：
影响因子：
4.7
通讯作者：
Topal,MD
中科院分区：
文献类型：
--
作者：
Rossi,SC;Conrad,M;Voigt,JM;Topal,MD
在N-甲基-N-硝基尿苷(MNU)处理的大鼠中，O6-甲基鸟嘌呤(O6-甲基G)被认为是导致H-rasproto癌基因突变激活的前突变损伤。对O_6-甲基G修复的研究主要集中在甲基转移酶上。潜在地，其他修复蛋白可能参与O6-甲基G的修复。我们研究了大肠杆菌UvrABC切除修复对构建在M13MP载体中的大鼠H-rasMNU部分序列中在大鼠H-rasMNU激活部位合成的O6-甲基G的影响。用寡核苷酸自选技术鉴定含有从含O6-甲基G链复制的DNA的子代噬菌体。我们发现，切除修复可以通过大鼠H-rasMNU激活位点的O6-甲基G来帮助防止突变。
O6-methylguanine (O6-methylG) is believed to be the pre-mutagenic lesion responsible for mutational activation of the H-rasproto-oncogene in rats treated withN-methyl-N-nitro-sourea (MNU). Research on the repair ofO6-methylG has primarily focused on the methyltransferases. Potentially, other repair proteins may be involved in repair ofO6-methylG. We have investigated the effect ofEscherichia coliUvrABC excision repair onO6-methylG synthesized at the rat H-rasMNU activation site in a partial rat H-rassequence constructed in an M13mp vector. An oligonucleotide self-selection technique was used to identify progeny phage containing DNA replicated from theO6-methylG-containing strand. We found that excision repair can help protect against mutation byO6-methylG at the rat H-rasMNU activation site.