Molecular analysis of duck hepatitis virus type 1 reveals a novel lineage close to the genus Parechovirus in the family Picornaviridae
Molecular analysis of duck hepatitis virus type 1 reveals a novel lineage close to the genus Parechovirus in the family Picornaviridae
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DOI：
10.1099/vir.0.81804-0
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发表时间：
2006-11-01
影响因子：
3.8
通讯作者：
Kim, Sun-Joong
中科院分区：
文献类型：
--
作者：
Kim, Min-Chul;Kwon, Yong-Kuk;Kim, Sun-Joong
鸭肝炎病毒1型（DHV-1）以前被归类为肠道病毒，主要是基于观察到的形态和病毒粒子的理化性质。已测定了DHV-1的两个毒株（DRL-62和R85952）的全核苷酸序列。除去poly（A）尾，基因组分别为7691和7690 nt，并且含有编码2249 aa的多聚蛋白的单个大开放阅读框。DHV-1的基因组的组织与小核糖核酸病毒科成员的基因组相同：5'非翻译区（UTR）-VP 0-VP 3-VP 1 - 2A 1 - 2A 2 -2B-2C-3A-3B-3C-3D-3' UTR。基因组和预测的多蛋白序列的分析揭示了几个不寻常的特征，包括缺乏预测的成熟切割的VPO，存在两个不相关的2A蛋白基序和3'非翻译区显着延长相比，任何其他小核糖核酸病毒。2A 1蛋白基序与口蹄疫病毒属的2A蛋白类型相关，相邻的2A 2蛋白与副肠孤病毒属中存在的2A蛋白类型相关。使用3D蛋白质序列的系统发育分析表明，这两个DHV-1株与副肠孤病毒属成员的关系比与其他小核糖核酸病毒的关系更密切。然而，这两种DHV-1毒株形成了一个单系群，与副肠孤病毒属的成员明显不同。
Duck hepatitis virus type 1 (DHV-1) was previously classified as an enterovirus, based primarily on observed morphology and physicochemical properties of the virion. The complete nucleotide sequences of two strains (DRL-62 and R85952) of DHV-1 have been determined. Excluding the poly(A) tail, the genomes are 7691 and 7690 nt, respectively, and contain a single, large open reading frame encoding a polyprotein of 2249 aa. The genome of DHV-1 is organized as are those of members of the family Picornaviridae: 5' untranslated region (UTR)-VPO-VP3-VP1 -2A1-2A2-2B-2C-3A-3B-3C-3D-3' UTR. Analysis of the genomic and predicted polyprotein sequences revealed several unusual features, including the absence of a predicted maturation cleavage of VPO, the presence of two unrelated 2A protein motifs and a 3' UTR extended markedly compared with that of any other picornavirus. The 2A1 protein motif is related to the 2A protein type of the genus Aphthovirus and the adjacent 2A2 protein is related to the 2A protein type present in the genus Parechovirus. Phylogenetic analysis using the 3D protein sequence shows that the two DHV-1 strains are related more closely to members of the genus Parechovirus than to other picornaviruses. However, the two DHV-1 strains form a monophyletic group, clearly distinct from members of the genus Parechovirus.