Immune lysis of normal human and paroxysmal nocturnal hemoglobinuria (PNH) red blood cells. 3. The membrane defects caused by complement lysis.
Immune lysis of normal human and paroxysmal nocturnal hemoglobinuria (PNH) red blood cells. 3. The membrane defects caused by complement lysis.
复制标题
正常人和阵发性夜间血红蛋白尿（PNH）红细胞的免疫裂解。 3。由补体裂解引起的膜缺陷。
DOI：
10.1084/jem.123.6.969
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发表时间：
1966-06-01
期刊：
影响因子：
--
通讯作者：
Humphrey JH
中科院分区：
文献类型：
--
作者：
Rosse WF;Dourmashkin R;Humphrey JH
1.用电子显微镜研究了补体和抗体作用下人红细胞膜上产生的缺陷。这些是圆形到略卵形的孔，周围有一个不规则的环，约20埃厚。如果使用人补体（不考虑用于致敏的抗体），孔的平均直径约为103 A，如果使用豚鼠补体，孔的平均直径约为88 A。2.在相同条件下，正常和PNH红细胞中的孔似乎是相同的。用酸化的正常血清裂解PNH细胞所产生的膜缺陷（Ham试验）与用特异性抗体裂解补体所产生的膜缺陷相同，表明补体无疑是这种裂解的原因。3.有证据表明，当人补体作用于用抗-I抗体致敏的人红细胞时，补体的每次完全激活导致产生一簇空穴。这与豚鼠补体对绵羊细胞的作用形成对比，绵羊细胞的每次激活都会导致一个单一的孔。4.对于正常细胞和PNH细胞，可以附着于人红细胞的抗-I抗体分子的最大数目（即抗原位点的最小数目）为约500，000。5. PNH细胞裂解过程中产生的孔数与正常细胞相同。当所有细胞被过量的C '裂解时，每个膜上平均存在约90，000个孔。当补体有限时，由于PNH细胞对C'的特殊敏感性，较大比例的PNH细胞被裂解，但每个裂解细胞上的孔数与被相同浓度的C'裂解的正常细胞相同。
1. The defects produced on the membrane of the human red blood cell by the action of complement and antibody have been studied by the use of the electron microscope. These are round to slightly ovoid holes and are surrounded by an irregular ring, about 20 A thick. The mean diameter of the holes is about 103 A if human complement is used (regardless of the antibody used for sensitization) and about 88 A if guinea pig complement is used. 2. The holes in normal and PNH red cells appear to be identical, under the same conditions. The membrane defects produced by lysis of PNH cells with acidified normal serum (the Ham's test) are identical to those produced by complement lysis with specific antibody, indicating that complement is undoubtedly the cause of such lysis. 3. Evidence is presented that when human complement acts on human red cells sensitized with anti-I antibody, each complete activation of complement leads to the production of a cluster of holes. This contrasts to the action of guinea pig complement, on sheep cells, each activation of which leads to a single hole. 4. The maximum number of anti-I antibody molecules which can attach to a human red cell (i.e. the minimum number of antigen sites) is about 500,000 for both normal and PNH cells. 5. The number of holes produced during lysis of the PNH cell is the same as that of the normal cell. When all cells are lysed by am excess of C', a mean of about 90,000 holes are present on each membrane. When complement is limited, a larger proportion of PNH cells are lysed due to their peculiar sensitivity to C' but the number of holes on each lysed cell is the same as for normal cells lysed by the same concentration of C'.