Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue.
Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue.
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DOI：
10.1016/j.chemosphere.2014.12.090
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发表时间：
2015-06
期刊：
影响因子：
8.8
通讯作者：
Xiaoyan Yan;Xianhui Hao;Qingli Nie;C. Feng;Hongwei Wang;Zilong Sun;R. Niu;Jundong Wang
中科院分区：
文献类型：
--
作者：
Xiaoyan Yan;Xianhui Hao;Qingli Nie;C. Feng;Hongwei Wang;Zilong Sun;R. Niu;Jundong Wang
长期过量的氟（F）摄入会破坏骨沉积和重塑活动的平衡，并与氟骨症有关。I型胶原蛋白，这是负责骨的稳定性和细胞的生物功能，可以被破坏过量的F摄入。在这项研究中，氟化钠（NaF）以150 mg L-1口服给大鼠60和120 d。用透射电镜观察了过量氟摄入对大鼠骨超微结构和胶原形态的影响。此外，我们还采用定量真实的时间（qRT）-PCR技术研究了氟摄入对大鼠骨组织中COL 1A 1和COL 1A 2表达水平的影响，以阐明氟诱导胶原蛋白损伤的分子机制。结果表明，氟影响了骨组织中Ⅰ型胶原的排列，并引起了骨组织超微结构的改变。同时，氟中毒组COL 1A 1和COL 1A 2 mRNA表达降低，COL I蛋白水平降低。我们的结论是，过量的F摄入对I型胶原的排列产生不利影响，并引起骨组织超微结构的变化。COL 1A 1 mRNA表达的降低和COL I蛋白水平的改变可能是氟暴露导致骨骼损伤的原因之一。
Long-term excessive fluoride (F) intake disrupts the balance of bone deposition and remodeling activities and is linked to skeletal fluorosis. Type I collagen, which is responsible for bone stability and cell biological functions, can be damaged by excessive F ingestion. In this study, Sodium fluoride (NaF) was orally administrated to rat at 150 mg L−1for 60 and 120 d. We examined the effects of excessive F ingestion on the ultrastructure and collagen morphology of bone in rats by using transmission electron microscopy (TEM). Furthermore, we investigated the effect of F consumption on the expression levels ofCOL1A1andCOL1A2in the bone tissues of rats by using quantitative real time (qRT)-PCR, to elucidate the molecular mechanisms of F-induced collagen protein damage. Our results showed that F affected collagen I arrangement and produced ultrastructural changes in bone tissue. Meanwhile, the mRNA expression ofCOL1A1andCOL1A2were reduced and the COL I protein levels decreased in the fluorosis group. We concluded that excessive F ingestion adversely affected collagen I arrangement and caused ultrastructural changes in bone tissue. ReducedCOL1A1mRNA expression and altered COL I protein levels may contribute to the skeletal damage resulting from F exposure.