The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.
The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica.
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DOI：
10.1111/j.1567-1364.2012.12008.x
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发表时间：
2013-02
影响因子：
3.2
通讯作者：
X. Zhao;Min Li;Yun-qing Li;Xiang-Dong Chen;Xiang-Dong Gao
中科院分区：
文献类型：
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作者：
X. Zhao;Min Li;Yun-qing Li;Xiang-Dong Chen;Xiang-Dong Gao
出芽酵母中的Tec1p对酿酒酵母的二态转变起重要作用。在这项研究中，我们发现了Tec1p的同源物YlTec1p，在远亲二态酵母脂性耶氏酵母。YlTec1p含有一个进化上保守的TEA/ATTS dna结合域。在酿酒葡萄球菌tec1Δ细胞中，YlTEC1的表达挽救了侵袭性生长缺陷，并激活了FLO11-lacZ报告基因，表明YlTec1p与Tec1p在功能上相关。然而，YlTEC1表达未能激活fr - lacz报告基因，这表明这两种转录因子是不同的。基于基因缺失和过表达研究，YlTEC1在脂肪瘤酵母向菌丝的转化中起负向作用。通过酵母单杂交实验，我们发现YlTec1p激活而不是抑制了Y. lipolytica中的基因表达，并且YlTec1p对Y. lipolytica中FLO11-lacZ的激活至关重要。此外，在菌丝生长过程中，YlTec1p定位于细胞核，其核定位减少。我们推测，YlTec1p可能在正常情况下调节一组靶基因的表达，这些基因可能阻止而不是促进脂肪瘤菌的菌丝发育。我们的研究还表明，YlTEC1可能不受cAMP-protein kinase A通路的很大程度上调节。
Tec1p in the budding yeast Saccharomyces cerevisiae is important for dimorphic transition. In this study, we identified a homologue of Tec1p, YlTec1p, in the distantly related dimorphic yeast Yarrowia lipolytica. YlTec1p contains an evolutionarily conserved TEA/ATTS DNA-binding domain. Expression of YlTEC1 in S. cerevisiae tec1Δ cells rescued the invasive growth defect and activated a FLO11-lacZ reporter, indicating that YlTec1p is functionally related to Tec1p. However, YlTEC1 expression failed to activate an FRE-lacZ reporter, suggesting that these two transcription factors are different. YlTEC1 plays a negative role in the yeast-to-hypha transition in Y. lipolytica based on gene deletion and overexpression studies. We show that YlTec1p activates rather than represses gene expression in Y. lipolytica by yeast one-hybrid assay, and YlTec1p is critical for the activation of FLO11-lacZ in Y. lipolytica. In addition, YlTec1p localized to the nucleus and its nuclear localization decreased during hyphal growth. We speculate that YlTec1p may normally regulate the expression of a set of target genes that may prevent rather than promote hyphal development in Y. lipolytica. Our study also suggests that YlTEC1 may not be largely regulated by the cAMP-protein kinase A pathway.