Non-thermal Plasma Exposure Rapidly Attenuates Bacterial AHL-Dependent Quorum Sensing and Virulence.
Non-thermal Plasma Exposure Rapidly Attenuates Bacterial AHL-Dependent Quorum Sensing and Virulence.
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DOI：
10.1038/srep26320
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发表时间：
2016-05-31
影响因子：
4.6
通讯作者：
Gilmore BF
中科院分区：
文献类型：
--
作者：
Flynn PB;Busetti A;Wielogorska E;Chevallier OP;Elliott CT;Laverty G;Gorman SP;Graham WG;Gilmore BF
大气压非热等离子体的抗微生物活性已被详尽地表征，然而，需要阐明细菌产生和利用的生物分子与短等离子体暴露之间的相互作用，以优化冷等离子体技术并将其转化为临床应用。本研究的特点是非热等离子体暴露对酰基高丝氨酸内酯（阿勒）依赖的群体感应（QS）的影响。AHLs的血浆暴露以剂量依赖性方式降低了这些分子在细菌报告菌株中引起QS应答的能力。短时间暴露（30-60 s）产生一系列能够引发QS反应的次级化合物，随后在较长时间暴露后完全丧失AHL依赖性信号传导。UPLC-MS分析证实了阿勒分子的时间依赖性降解及其转化为一系列副产物。等离子体暴露的AHLs的FT-IR分析突出了OH基团的出现。使用标准体内模型检查AHL暴露于血浆的体内评估。用rhlI/lasI突变体PAO-MW 1与等离子体处理的N-丁酰-高丝氨酸内酯和n-（3-氧代-十二烷酰基）-高丝氨酸内酯一起注射莴苣叶，表现出明显的毒力衰减。这项研究强调了大气压非热等离子体修饰和降解阿勒自身诱导物的能力，从而减弱了铜绿假单胞菌中的QS依赖性毒力。
The antimicrobial activity of atmospheric pressure non-thermal plasma has been exhaustively characterised, however elucidation of the interactions between biomolecules produced and utilised by bacteria and short plasma exposures are required for optimisation and clinical translation of cold plasma technology. This study characterizes the effects of non-thermal plasma exposure on acyl homoserine lactone (AHL)-dependent quorum sensing (QS). Plasma exposure of AHLs reduced the ability of such molecules to elicit a QS response in bacterial reporter strains in a dose-dependent manner. Short exposures (30–60 s) produce of a series of secondary compounds capable of eliciting a QS response, followed by the complete loss of AHL-dependent signalling following longer exposures. UPLC-MS analysis confirmed the time-dependent degradation of AHL molecules and their conversion into a series of by-products. FT-IR analysis of plasma-exposed AHLs highlighted the appearance of an OH group. In vivo assessment of the exposure of AHLs to plasma was examined using a standard in vivo model. Lettuce leaves injected with the rhlI/lasI mutant PAO-MW1 alongside plasma treated N-butyryl-homoserine lactone and n-(3-oxo-dodecanoyl)-homoserine lactone, exhibited marked attenuation of virulence. This study highlights the capacity of atmospheric pressure non-thermal plasma to modify and degrade AHL autoinducers thereby attenuating QS-dependent virulence in P. aeruginosa.