EXPRESSION OF INTERMEDIATE FILAMENT PROTEINS IN FETAL AND ADULT HUMAN-LUNG TISSUES
EXPRESSION OF INTERMEDIATE FILAMENT PROTEINS IN FETAL AND ADULT HUMAN-LUNG TISSUES
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DOI：
10.1111/j.1432-0436.1989.tb00821.x
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发表时间：
1989-05-01
期刊：
影响因子：
2.9
通讯作者：
RAMAEKERS, FCS
中科院分区：
文献类型：
--
作者：
BROERS, JLV;DELEIJ, L;RAMAEKERS, FCS
使用（链特异性）单克隆抗体描述了胎儿和正常或非病理性成人肺组织中中间丝蛋白的表达模式。在发育的早期阶段（妊娠9-10周和25周），在支气管上皮细胞中仅检测到所谓的简单细胞角蛋白，如细胞角蛋白7（少量）、8、18和19。在发育的后期，细胞角蛋白的表达模式变得更加复杂。细胞角蛋白7阳性的支气管细胞数量增加，但支气管上皮中的基底细胞保持阴性。然而，这些后一种细胞在妊娠晚期显示细胞角蛋白14的表达。发育中的肺泡上皮细胞表达细胞角蛋白7、8、18和19。在成人支气管上皮细胞角蛋白4（不同量），7，8，13（少量），14，18和19可以检测到，细胞角蛋白7，8和18的主要表达在柱状细胞和细胞角蛋白14的主要表达在基底细胞。波形蛋白在所有间充质组织中检测到。此外，胎儿肺表达波形蛋白的支气管上皮细胞，但随着年龄的增长，在较小的程度上，导致波形蛋白的表达在出生时只有少数分散的支气管细胞。在成人支气管上皮中，波形蛋白的表达也见于部分基底和柱状上皮细胞。存在于肺平滑肌细胞中的结蛋白丝似乎随着年龄的增长而改变其蛋白质结构。在发育的早期阶段，血管周围的平滑肌细胞部分地与一些细胞角蛋白抗体和多克隆结蛋白抗体反应。然而，在妊娠第9-10周和第25周，结蛋白单克隆抗体不与血管平滑肌细胞反应，而仅与支气管周围的平滑肌细胞反应。随着年龄的增长，细胞角蛋白抗体与血管中平滑肌细胞的反应性降低，而与单克隆结蛋白抗体的反应性增加。我们的研究结果表明，在分化过程中，中间丝表达模式发生了深刻的变化，在不同类型的细胞发育肺。
The expression patterns of intermediate filament proteins in fetal and normal or nonpathological adult human lung tissues are described using (chain-specific) monoclonal antibodies. In early stages of development (9-10 weeks and 25 weeks of gestation) only so-called simple cytokeratins such as cytokeratins 7 (minor amounts), 8, 18 and 19 are detected in bronchial epithelial cells. At later stages of development, the cytokeratin expression patterns become more complex. The number of bronchial cells positive for cytokeratin 7 increases, but basal cells in the bronchial epithelium remain negative. These latter cells show, however, expression of cytokeratin 14 in the third trimester of gestation. Developing alveolar epithelial cells express cytokeratins 7, 8, 18 and 19. In adult human bronchial epithelium cytokeratins 4 (varying amounts), 7, 8, 13 (minor amounts), 14, 18 and 19 can be detected, with the main expression of cytokeratins 7, 8, and 18 in columnar cells and the main expression of cytokeratin 14 in basal cells. Vimentin is detected in all mesenchymal tissues. In addition, fetal lung expresses vimentin in bronchial epithelium, however, to a lesser extent with increasing age, resulting in the expression of vimentin in only few scattered bronchial cells at birth. Also in adult bronchial epithelium the expression of vimentin is noticed in part of the basal and columnar epithelial cells. Desmin filaments, present in smooth muscle cells of the lung, appear to alter their protein structure with age. In early stages of development smooth muscle cells surrounding blood vessels are partly reactive with some cytokeratin antibodies and with a polyclonal desmin antibody. At week 9-10 and week 25 of gestation a monoclonal antibody to desmin, however, is not reactive with blood vessel smooth muscle cells but is only reactive with smooth muscle cells surrounding bronchi. With increasing age the reactivity of cytokeratin antibodies with smooth muscle cells in blood vessels decreases, while the reactivity with the monoclonal desmin antibody increases. Our results show that during differentiation profound changes in the intermediate filament expression patterns occur in the different cell types of the developing lung.