Simple Plex(™) : A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines.
Simple Plex(™) : A Novel Multi-Analyte, Automated Microfluidic Immunoassay Platform for the Detection of Human and Mouse Cytokines and Chemokines.
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DOI：
10.1111/aji.12512
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Mor G
中科院分区：
文献类型：
--
作者：
Aldo P;Marusov G;Svancara D;David J;Mor G
体液或细胞制剂中蛋白质的定量测量对于生物标志物的评价或复杂细胞过程的研究至关重要。虽然免疫测定是迄今为止使用的最常见的定量方法，但它们对于评价多种蛋白质并不实用。微流控技术允许在微通道内固定蛋白质和生物分子的精细空间控制，消除竞争分析物之间的交叉反应，并允许快速和灵敏地检测多种应用的靶抗原。我们报告了用于检测和定量来自人类和小鼠样品的细胞因子和趋化因子的Simple Risk ™平台的表征和验证。使用来自ProteinSimple的Simple ELISA盒测定细胞因子和趋化因子表达水平。血清样品获自Yale Biorepository。我们的数据表明，Simple Plex获得的结果与ELISA和Luminex等传统免疫测定法之间具有良好的相关性。我们描述了简单微流控的表征和验证，简单微流控是一种新型的多分析物自动化微流控平台，可以评估人类和小鼠生物样品中的细胞因子和趋化因子。与传统方法相比，简单重复性分析法在样品体积要求低、灵敏度和动态范围、变异系数和重现性方面具有显著优势。
Quantitative measurement of proteins in bodily fluids or cellular preparations is critical for the evaluation of biomarkers or the study of complex cellular processes. While immunoassays are the most common quantitative approach used so far, they are not practical for the evaluation of multiple proteins. Microfluidic technology allows a fine spatial control in immobilizing proteins and biomolecules inside microchannels, eliminating cross‐reactivity between competing analytes, and allowing rapid and sensitive detection of targeted antigens for multiple applications. We report the characterization and validation of the Simple Plex™ platform for the detection and quantification of cytokines and chemokines from human and mouse samples. Cytokine and chemokine expression levels were determined using Simple Plex cartridges from ProteinSimple. Serum samples were obtained from the Yale Biorepository. Our data demonstrate an excellent correlation between the results obtained with Simple Plex and conventional immunoassays such as ELISA and Luminex. We describe the characterization and validation of Simple Plex, a novel multi‐analyte, automated microfluidic platform that allows the evaluation of cytokines and chemokines from human and mice biological samples. Simple Plex showed significant advantages over traditional approaches in terms of low sample volume requirements, sensitivity and dynamic range, coefficient of variation, and reproducibility.
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影响因子：
9.3
作者：
Ellington AA;Kullo IJ;Bailey KR;Klee GG
通讯作者：
Klee GG
影响因子：
9.3
作者：
Ellington, Allison A.;Kullo, Iftikhar J.;Klee, George G.
通讯作者：
Klee, George G.
影响因子：
2.9
作者：
Racicot, Karen E.;Wuensche, Vera;Mor, Gil
通讯作者：
Mor, Gil
DOI：
10.1111/j.1600-0897.2010.00908.x
发表时间：
2011-02-01
影响因子：
3.6
作者：
Cardenas, Ingrid;Mor, Gil;Means, Robert E.
通讯作者：
Means, Robert E.
影响因子：
3.8
作者：
Wong, Hui-Lee;Pfeiffer, Ruth M.;Rabkin, Charles S.
通讯作者：
Rabkin, Charles S.