USE OF A NEW CD4-POSITIVE HELA-CELL CLONE FOR DIRECT QUANTITATION OF INFECTIOUS HUMAN-IMMUNODEFICIENCY-VIRUS FROM BLOOD-CELLS OF AIDS PATIENTS
USE OF A NEW CD4-POSITIVE HELA-CELL CLONE FOR DIRECT QUANTITATION OF INFECTIOUS HUMAN-IMMUNODEFICIENCY-VIRUS FROM BLOOD-CELLS OF AIDS PATIENTS
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DOI：
10.1093/infdis/163.1.64
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发表时间：
1991-01-01
影响因子：
6.4
通讯作者：
GRIFFIN, DE
中科院分区：
文献类型：
--
作者：
CHESEBRO, B;WEHRLY, K;GRIFFIN, DE
一种新的 CD4 阳性 HeLa 细胞系（克隆 1022）对从 AIDS 患者中分离出来的人类免疫缺陷病毒（HIV）具有更高的敏感性，可以使大多数分离株的 HIV 感染滴度达到与使用正常人植物血凝素（PHA）刺激的淋巴细胞培养物观察到的水平相同。 通过使用该克隆与局灶性免疫分析 (FIA)，在 15% 的血清阳性无症状患者和 23% 的 AIDS 患者中检测到产生 HIV 的外周血单核细胞 (PBMC)，频率为 2 X 10(4) 至 3 X 10(6) PBMC 中的 1 个。 初级 FIA 检测到的 HIV 与低 CD4 阳性细胞计数相关。 与 PHA 母细胞共培养中的 HIV 激活导致共培养后 3-4 天开始释放 HIV 的细胞数量增加。 与 PHA 母细胞共培养后 HIV 分离的高发生率相比，直接 FIA 检测出 HIV 的发生率较低，这提供了定量感染性数据，表明 HIV 在大多数 AIDS 患者的 PBMC 中处于潜伏或低表达状态。
A new CD4-positive HeLa cell line (clone 1022) with increased sensitivity for human immunodeficiency virus (HIV) isolated derived from AIDS patients could titer infectivity of HIV from most isolates at a level equal to that observed using normal human phytohemagglutinin (PHA)-stimulated lymphocyte cultures. By use of this clone with a focal immunoassay (FIA), peripheral blood mononuclear cells (PBMC) producing HIV were detected in 15% of seropositive asymptomatic patients and 23% of AIDS patients at a frequency of 1 in 2 X 10(4) to 3 X 10(6) PBMC. HIV detection by primary FIA correlated with low CD4-positive cells counts. HIV activation in cocultures with PHA blasts resulted in increasing numbers of cells releasing HIV starting 3-4 days after cocultivation. The low incidence of HIV detection by direct FIA compared with the high incidence if HIV isolation after cocultivation with PHA blasts provided quantiative infectivity data suggesting that HIV was in a state of latency or low expression in most PBMC of AIDS patients.