Phospholipase A activity associated with the growth of Rickettsia prowazekii in L929 cells.
Phospholipase A activity associated with the growth of Rickettsia prowazekii in L929 cells.
复制标题
磷脂酶 A 活性与 L929 细胞中普氏立克次体的生长相关。
DOI：
10.1128/iai.57.1.36-40.1989
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Daugherty,RM
中科院分区：
文献类型：
--
作者：
Winkler,HH;Daugherty,RM
Cultured L929 cells infected with Rickettsia prowazekii had a greatly increased rate of hydrolysis of fatty acid from the oleic acid-radiolabeled phospholipids of the host cell membranes. The incorporation of fatty acid into phospholipid in an infected cell was only moderately inhibited relative to a mock-infected cell. Thus, even if the release of fatty acid from phospholipid represented a steady state between hydrolysis and resynthesis of phospholipids, the increase in release of fatty acid was due principally to increased phospholipase A activity. The increased rate of hydrolysis did not occur only late in the rickettsial infection; this activity began early in infection and continued throughout the course of infection. The addition of tetracycline or chloramphenicol (antibiotics which inhibit rickettsial protein synthesis) to the infected cells caused a rapid and total abatement of this increased rate of phospholipid hydrolysis. In contrast, high concentrations of penicillin affected the morphology of the intracellular rickettsiae, but did not inhibit the phospholipase activity. This phospholipase A activity clearly damages the host cell during the rickettsial infection and may represent the activity by which R. prowazekii escapes from the host cell.
