LOCATION OF TRANSCRIPTIONAL CONTROL SIGNALS AND TRANSFER-RNA SEQUENCES IN TORULOPSIS-GLABRATA MITOCHONDRIAL-DNA
LOCATION OF TRANSCRIPTIONAL CONTROL SIGNALS AND TRANSFER-RNA SEQUENCES IN TORULOPSIS-GLABRATA MITOCHONDRIAL-DNA
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DOI：
10.1002/j.1460-2075.1985.tb03652.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
SRIPRAKASH, KS
中科院分区：
文献类型：
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作者：
CLARKWALKER, GD;MCARTHUR, CR;SRIPRAKASH, KS
通过对裸鼠19 kbp线粒体DNA中分离大基因的9个区域的序列测定，鉴定出23个tRNA基因，并识别出2种与线粒体基因组表达有关的短重复序列。2个短重复序列为非核苷酸基序5′-TATAAGTAA-3′和十二核苷酸基序5′-TATAATATTCTT-3′。RNA序列测定表明，小RNA和大RNA的初级转录本开始于非核苷酸序列的倒数3‘’腺嘌呤。在f-蛋氨酸、苯丙氨酸、亮氨酸、酪氨酸和甘氨酸trna、细胞色素氧化酶亚基2和atp酶亚基9上游的DNA序列中也发现了该基序。在大基因之间的9个区域中，每个区域至少发现一次十二核核苷酸序列。小rna和大rna的3”末端的测定显示它们位于十二核苷酸序列下游的8和23个核苷酸。该基序可能参与多顺反子转录本的信号处理。这些转录物被用来解释细胞色素b、细胞色素氧化酶亚基1和3的mRNA的产生，以及缺乏相邻上游非核苷酸转录起始信号序列的atp酶亚基8和6基因的联合mRNA的产生。tRNA序列上的多顺反子转录本的加工也与成熟mrna的形成有关。从tRNA基因相对于非核苷酸基序的位置来看，这些基因簇似乎与细胞色素氧化酶亚基1和3的下游序列共转录。
Determination of sequences from the 9 regions separating the large genes in the 19-kbp mitochondrial DNA from T. glabrata led to the identification of 23 tRNA genes and to the recognition of 2 types of short repeated sequence implicated in mitochondrial genome expression. The 2 short repeated sequences are a nonanucleotide motif, 5''-TATAAGTAA-3'' and a dodecanucleotide motif, 5''-TATAATATTCTT-3''. RNA sequence determination showed that primary transcripts of the small and large rRNAs commence at the 3'' penultimate adenine of the nonanucleotide sequence. This motif has also been found in the DNA sequence upstream from f-methionine, phenylalanine, leucine, tyrosine and glycine tRNAs, cytochrome oxidase subunit 2 and ATPase subunit 9. The dodecanucleotide sequence is found at least once in each of the 9 regions between the large genes. Determination of the 3'' ends of the small and large rRNAs showed their location at 8 and 23 nucleotides downstream from the dodecanucleotide sequence. This motif is probably involved in signaling processing of polycistronic transcripts. Such transcripts are invoked to account for the production of mRNAs for cytochrome b, cytochrome oxidase subunits 1 and 3, and the joint mRNA for ATPase subunits 8 and 6 genes that lack an adjacent upstream nonanucleotide transcription initiation signal sequence. Processing of polycistronic transcripts at tRNA sequences is also implicated in the formation of mature mRNAs. From the position of tRNA genes relative to the nonanucleotide motif it appears that clusters of these genes are co-transcribed with downstream sequences for cytochrome oxidase subunits 1 and 3.