Fast NMR Data Acquisition - Beyond the Fourier Transform
Fast NMR Data Acquisition - Beyond the Fourier Transform
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快速 NMR 数据采集 - 超越傅里叶变换
DOI：
10.1039/9781782628361-00267
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Bostock M
中科院分区：
文献类型：
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作者：
Bostock M
沿着X射线晶体学，NMR光谱学是唯一可用于研究分子结构的原子分辨率技术。多年来，许多发展显着增加了生物分子NMR研究可以应用的尺寸限制，特别是包括TROSY技术，1先进的标记策略，包括选择性甲基质子化2和甲基定向实验，3以及NMR硬件的持续改进。这些进展使NMR光谱探测越来越苛刻的应用，如蛋白质复合物4，5和更大的膜蛋白。6，7在大多数情况下，多维NMR实验已被证明是必不可少的，以实现主链和侧链的分配，以获得
Along with X-ray crystallography, NMR spectroscopy is the only atomic resolution technique available to study molecular structure. Over the years, many developments have significantly increased the size-limit to which biomolecular NMR studies can be applied, including in particular the TROSY technique, 1 advanced labelling strategies including selective methyl protonation2 and methyl-directed experiments, 3 as well as continuing improvements in NMR hardware. Such advances have enabled NMR spectroscopy to probe increasingly demanding applications, such as protein complexes4, 5 and larger membrane proteins. 6, 7 In the majority of cases, multidimensional NMR experiments have proved indispensable to achieve backbone and side chain assignments, to acquire