Mechanism and function of poleward flux in Xenopus extract meiotic spindles
Mechanism and function of poleward flux in Xenopus extract meiotic spindles
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DOI：
10.1098/rstb.2004.1616
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发表时间：
2005-03-29
影响因子：
6.3
通讯作者：
Mitchison, TJ
中科院分区：
文献类型：
--
作者：
Mitchison, TJ
在非洲爪蟾提取物减数分裂纺锤体中，微管不断地滑向它们的负端，这一过程称为向极流。本文讨论了极向流机制的研究进展，以及极向流在纺锤体组织和染色体作用力产生中的作用。双极组织所需的通量和有丝分裂驱动蛋白Eg5抑制通量，这表明通量的滑动力是由Eg5推动反平行微管分开。在纺锤体组织中，通量的一个重要功能可能是将在染色质上成核的负末端运输到极点。通过将微管拉过着丝粒上的附着位点，通量可以在中期染色体上产生向极的力。
In Xenopus extract meiotic spindles, microtubules slide continuously towards their minus ends, a process called poleward flux. This article discusses recent progress in determining the mechanism of poleward flux, and its functions in spindle organization and generating force on chromosomes. Bipolar organization is required for flux and inhibition of the mitotic kinesin Eg5 inhibits flux, suggesting the sliding force for flux is generated by Eg5 pushing anti-parallel microtubules apart. An important function of flux in spindle organization may be to transport minus ends nucleated at chromatin towards the pole. By pulling microtubules through attachment sites at kinetochores, flux may generate poleward force on metaphase chromosomes.