Structural basis for the nonlinear mechanics of fibrin networks under compression.
Structural basis for the nonlinear mechanics of fibrin networks under compression.
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DOI：
10.1016/j.biomaterials.2014.04.056
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发表时间：
2014-08
期刊：
影响因子：
14
通讯作者：
Alber, Mark S.
中科院分区：
文献类型：
--
作者：
Kim, Oleg V.;Litvinov, Rustem I.;Weisel, John W.;Alber, Mark S.
纤维蛋白是一种形成3D丝状网络的蛋白质聚合物，是保护性生理性血栓和危及生命的病理性血栓的主要结构成分。它在伤口愈合、组织再生等方面发挥着重要作用，被广泛应用于外科手术、组织工程等领域。这项研究的目的是建立纤维蛋白网络在压缩负荷下的结构变化和力学反应之间的相关性。流变学测量表明，在动态压缩下，纤维蛋白网络的粘弹性性质发生了非线性变化，导致了网络的软化和急剧硬化。反复加压/减压增强纤维蛋白凝块硬化。结合纤维蛋白网络流变学和同步共聚焦显微镜，提供了压缩纤维蛋白网络非线性粘弹性背后的结构调制的直接证据。纤维蛋白凝块对压缩的反应软化与纤维弯曲和弯曲密切相关，而硬化与纤维蛋白网络致密有关。我们的结果表明，在纤维蛋白网络经历压缩变形的过程中，伴随着结构变化和解释非线性力学响应的熵和焓机制之间存在复杂的相互作用。这些发现为纤维蛋白凝块的结构力学提供了新的见解，并可用于设计具有调节粘弹性性能的纤维蛋白生物材料。
Fibrin is a protein polymer that forms a 3D filamentous network, a major structural component of protective physiological blood clots as well as life threatening pathological thrombi. It plays an important role in wound healing, tissue regeneration and is widely employed in surgery as a sealant and in tissue engineering as a scaffold. The goal of this study was to establish correlations between structural changes and mechanical responses of fibrin networks exposed to compressive loads. Rheological measurements revealed nonlinear changes of fibrin network viscoelastic properties under dynamic compression, resulting in network softening followed by its dramatic hardening. Repeated compression/decompression enhanced fibrin clot stiffening. Combining fibrin network rheology with simultaneous confocal microscopy provided direct evidence of structural modulations underlying nonlinear viscoelasticity of compressed fibrin networks. Fibrin clot softening in response to compression strongly correlated with fiber buckling and bending, while hardening was associated with fibrin network densification. Our results suggest a complex interplay of entropic and enthalpic mechanisms accompanying structural changes and accounting for the nonlinear mechanical response in fibrin networks undergoing compressive deformations. These findings provide new insight into the fibrin clot structural mechanics and can be useful for designing fibrin-based biomaterials with modulated viscoelastic properties.
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DOI：
10.1073/pnas.86.4.1113
发表时间：
1989-02-01
影响因子：
11.1
作者：
MOSESSON, MW;SIEBENLIST, KR;DIORIO, JP
通讯作者：
DIORIO, JP
液晶弹性体中的动态自动化。
影响因子：
16.6
作者：
通讯作者：
--
DOI：
10.1021/jp807749f
发表时间：
2009-03-26
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Kang H;Wen Q;Janmey PA;Tang JX;Conti E;MacKintosh FC
通讯作者：
MacKintosh FC
影响因子：
3.3
作者：
JANMEY, PA;AMIS, EJ;FERRY, JD
通讯作者：
FERRY, JD
影响因子：
41.2
作者：
Janmey, Paul A.;Mccormick, Margaret E.;Mackintosh, Fred C.
通讯作者：
Mackintosh, Fred C.